ArticleJournal of bacteriology2012
Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Innate immune response to Streptococcus suis infection: advances and future challenges.Veterinary research · 2026Review
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- Insight into the role of Streptococcus suis zinc metalloprotease C from the new serotype causing meningitis in piglets.BMC veterinary research · 2024Article
- How Streptococcus suis escapes antibiotic treatments.Veterinary research · 2022Review
- Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions.Archives of microbiology · 2021Article
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- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response ofFrontiers in cellular and infection microbiology · 2018Article
- Inhibition ofFrontiers in pharmacology · 2018Article
- A serine/threonine phosphatase 1 ofJournal of veterinary science · 2017Article
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- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of ZoonoticFrontiers in cellular and infection microbiology · 2017Article
- Mac Protein is not an Essential Virulence Factor for the Virulent Reference Strain Streptococcus suis P1/7.Current microbiology · 2017Article
- Article
- Neutrophils in host defense: new insights from zebrafish.Journal of leukocyte biology · 2015Review
- Novel variant serotype of streptococcus suis isolated from piglets with meningitis.Applied and environmental microbiology · 2015Article
- Identification of Novel Laminin- and Fibronectin-binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2.Frontiers in cellular and infection microbiology · 2015Article
- The identification of six novel proteins with fibronectin or collagen type I binding activity from Streptococcus suis serotype 2.Journal of microbiology (Seoul, Korea) · 2014Article
- Two novel functions of hyaluronidase from Streptococcus agalactiae are enhanced intracellular survival and inhibition of proinflammatory cytokine expression.Infection and immunity · 2014Article
- The Streptococcus suis transcriptional landscape reveals adaptation mechanisms in pig blood and cerebrospinal fluid.RNA (New York, N.Y.) · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus suis serotype 2 (S. suis 2) is an important swine and human pathogen responsible for septicemia and meningitis. A novel gene, designated atl and encoding a major autolysin of S. suis 2 virulent strain HA9801, was identified and characterized in this study. The Atl protein contains 1,025 amino acids with a predicted molecular mass of 113 kDa and has a conserved N-acetylmuramoyl-l-alanine amidase domain. Recombinant Atl was expressed in Escherichia coli, and its bacteriolytic and fibronectin-binding activities were confirmed by zymography and Western affinity blotting. Two bacteriolytic bands were shown in the sodium dodecyl sulfate extracts of HA9801, while both were absent from the atl inactivated mutant. Cell chains of the mutant strain became longer than that of the parental strain. In the autolysis assay, HA9801 decreased to 20% of the initial optical density (OD) value, while the mutant strain had almost no autolytic activity. The biofilm capacity of the atl mutant was reduced ∼30% compared to the parental strain. In the zebrafish infection model, the 50% lethal dose of the mutant strain was increased up to 5-fold. Furthermore, the adherence to HEp-2 cells of the atl mutant was 50% less than that of the parental strain. Based on the functional analysis of the recombinant Atl and observed effects of atl inactivation on HA9801, we conclude that Atl is a major autolysin of HA9801. It takes part in cell autolysis, separation of daughter cells, biofilm formation, fibronectin-binding activity, cell adhesion, and pathogenesis of HA9801.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.