ArticleThe Journal of allergy and clinical immunology2012
Airway epithelial cells from asthmatic children differentially express proremodeling factors.
Article in The Journal of allergy and clinical immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers, 1 of them a synthesis that pooled it.
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Who cites it
81 citing papers in PubMed, 1 synthesis or guideline pooled it, 172 citations in OpenAlex.
- Exposure to violence, chronic stress, nasal DNA methylation, and atopic asthma in children.Pediatric pulmonology · 2021Pooled it
- Bronchial epithelial transcriptome reveals dysregulated interferon and inflammatory responses to rhinovirus in exacerbation-prone pediatric asthma.JCI insight · 2025Article
- IL-13 Modulates Antiviral Effector and Proinflammatory Pathways in Rhinovirus-Infected Pediatric Bronchial Epithelium.bioRxiv : the preprint server for biology · 2025Article
- Transcriptomic Profiling of Bronchial Epithelium Reveals Dysregulated Interferon and Inflammatory Responses to Rhinovirus in Exacerbation-Prone Pediatric Asthma.bioRxiv : the preprint server for biology · 2025Article
- Where eye meets body part 2: uniting allergy pathways in ocular and atopic disease - T cells take the lead.Current opinion in allergy and clinical immunology · 2025Review
- Inflammation of the nasal mucosa is associated with susceptibility to experimental pneumococcal challenge in older adults.Mucosal immunology · 2024Article
- Immunological factors, important players in the development of asthma.BMC immunology · 2024Review
- Immunologic aspects of asthma: from molecular mechanisms to disease pathophysiology and clinical translation.Frontiers in immunology · 2024Review
- Early Prediction of Asthma.Journal of clinical medicine · 2023Review
- Rhinovirus C causes heterogeneous infection and gene expression in airway epithelial cell subsets.Mucosal immunology · 2023Article
- Rhinovirus infection of the airway epithelium enhances mast cell immune responses via epithelial-derived interferons.The Journal of allergy and clinical immunology · 2023Article
- Review
- Nasal TSLP and periostin in infants with severe bronchiolitis and risk of asthma at 4 years of age.Respiratory research · 2023Observational
- How does cigarette smoking affect airway remodeling in asthmatics?Tobacco induced diseases · 2023Review
- Airway epithelial interferon response to SARS-CoV-2 is inferior to rhinovirus and heterologous rhinovirus infection suppresses SARS-CoV-2 replication.Scientific reports · 2022Article
- Asthmatic Eosinophils Alter the Gene Expression of Extracellular Matrix Proteins in Airway Smooth Muscle Cells and Pulmonary Fibroblasts.International journal of molecular sciences · 2022Article
- SIRT3 regulates bronchial epithelium apoptosis and aggravates airway inflammation in asthma.Molecular medicine reports · 2022Article
- Effects of Different Intervention Factors on Vascular Endothelial Growth Factor-Induced Human Airway Smooth Muscle Cell Migration.Canadian respiratory journal · 2022Article
- Serum Periostin Predicts Wheezing Exacerbation: A Prospective Study in Preschool Children with Recurrent Wheezing.International archives of allergy and immunology · 2022Article
- Human bronchial epithelial cells from patients with asthma have an altered gene expression profile.ERJ open research · 2022Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundThe airway epithelium can express factors that drive subepithelial airway remodeling. TGF-β2, vascular epithelial growth factor (VEGF), a disintegrin and metalloprotease 33 (ADAM33), and periostin are hypothesized to be involved in subepithelial remodeling and are overexpressed in adult asthmatic airways. Epidemiologic data suggest that lung function deficits in asthmatic patients are acquired in childhood.
objectivesWe sought to determine whether airway epithelial cells (AECs) from asthmatic children differentially express TGF-β2, VEGF, ADAM33, or periostin compared with cells from atopic nonasthmatic and healthy children intrinsically or in response to IL-4/IL-13 stimulation.
methodsBronchial and nasal epithelial cells were obtained from brushings from well-characterized asthmatic (n = 16), atopic nonasthmatic (n = 9), and healthy (n = 15) children after achievement of anesthesia for elective procedures. After differentiation at an air-liquid interface (ALI) for 3 weeks, conditioned media were sampled and RNA was extracted from unstimulated and IL-4/IL-13-stimulated cultures. TGF-β2 and VEGF levels were measured with ELISA. ADAM33 and periostin expression was assessed by using real-time PCR.
resultsTGF-β2 and VEGF production was significantly greater in bronchial and nasal ALI cultures from asthmatic children than in cultures from atopic nonasthmatic and healthy children. TGF-β2 levels increased significantly in asthmatic cultures after IL-4/IL-13 stimulation. Within-subject correlation between nasal and bronchial ALI production of TGF-β2 (r = 0.64, P = .001) and VEGF (r = 0.73, P < .001) was good. Periostin expression was 3.7-fold higher in bronchial cells (P < .001) and 3.9-fold higher in nasal cells (P < .004) from asthmatic children than in cells from atopic nonasthmatic or healthy children. ADAM33 was not differentially expressed by AECs from asthmatic patients compared with that from cells from atopic nonasthmatic or healthy children.
conclusionAECs from asthmatic children differentially express TGF-β2, VEGF, and periostin compared with cells from atopic nonasthmatic and healthy children. Nasal epithelial cells might be a suitable surrogate for bronchial cells that could facilitate investigation of the airway epithelium in future longitudinal pediatric studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.