ArticleBiochimie2012
Testing the faux-UTR model for NMD: analysis of Upf1p and Pab1p competition for binding to eRF3/Sup35p.
Article in Biochimie, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed.
- Nonsense-mediated mRNA decay and associated splicing patterns in neurodevelopmental disorders.Frontiers in molecular biosciences · 2026Review
- Nonsense-mediated mRNA decay: a key regulatory system engaged in cancer.Cell communication and signaling : CCS · 2025Review
- Nonsense-Mediated mRNA Decay: Mechanisms and Recent Implications in Cardiovascular Diseases.Cells · 2025Review
- Cytoplasmic mRNA decay and quality control machineries in eukaryotes.Nature reviews. Genetics · 2025Review
- Characterization of the mIF4G Domains in the RNA Surveillance Protein Upf2p.Current issues in molecular biology · 2023Article
- The long and short of EJC-independent nonsense-mediated RNA decay.Biochemical Society transactions · 2023Review
- An improved imaging system that corrects MS2-induced RNA destabilization.Nature methods · 2022Article
- Deciphering the nonsense-mediated mRNA decay pathway to identify cancer cell vulnerabilities for effective cancer therapy.Journal of experimental & clinical cancer research : CR · 2021Review
- Molecular Determinants and Specificity of mRNA with Alternatively-Spliced UPF1 Isoforms, Influenced by an Insertion in the 'Regulatory Loop'.International journal of molecular sciences · 2021Article
- UPF1-Mediated RNA Decay-Danse Macabre in a Cloud.Biomolecules · 2020Review
- Nonsense-mediated mRNA decay: The challenge of telling right from wrong in a complex transcriptome.Wiley interdisciplinary reviews. RNA · 2019Review
- The splicing factor Sf3b1 regulates erythroid maturation and proliferation via TGFβ signaling in zebrafish.Blood advances · 2019Article
- Quality and quantity control of gene expression by nonsense-mediated mRNA decay.Nature reviews. Molecular cell biology · 2019Review
- Nonsense-mediated mRNA decay: novel mechanistic insights and biological impact.Wiley interdisciplinary reviews. RNA · 2016Review
- Review
- Nonsense-mediated RNA decay--a switch and dial for regulating gene expression.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.Annual review of genetics · 2015Review
- Interaction between the poly(A)-binding protein Pab1 and the eukaryotic release factor eRF3 regulates translation termination but not mRNA decay in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2015Article
- Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1.Nucleic acids research · 2014Article
- The dharma of nonsense-mediated mRNA decay in mammalian cells.Molecules and cells · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that accelerates the degradation of mRNAs containing premature translation termination codons. This quality control pathway depends on the NMD-specific factors, Upf1p, Upf2p/Nmd2p, and Upf3p, as well as the two release factors, eRF1 and eRF3 (respectively designated Sup45p and Sup35p in yeast). NMD activation is also enabled by the absence of the poly(A)-binding protein, Pab1p, downstream of a termination event. Since Sup35p interacts with both Upf1p and Pab1p we considered the possibility that differential binding of the latter factors to Sup35p may be a critical determinant of NMD sensitivity for an mRNA. Here we describe three approaches to assess this hypothesis. First, we tethered fragments or mutant forms of Sup35p downstream of a premature termination codon in a mini-pgk1 nonsense-containing mRNA and showed that the inhibition of NMD by tethered Sup35p does not depend on the domain necessary for the recruitment of Pab1p. Second, we examined the Sup35p interaction properties of Upf1p and Pab1p in vitro and showed that these two proteins bind differentially to Sup35p. Finally, we examined competitive binding between the three proteins and observed that Upf1p inhibits Pab1p binding to Sup35p whereas the interaction between Upf1p and Sup35p is relatively unaffected by Pab1p. These data indicate that the binding of Upf1p and Pab1p to Sup35p may be more complex than anticipated and that NMD activation could involve more than just simple competition between these factors. We conclude that activation of NMD at a premature termination codon is not solely based on the absence of Pab1p and suggest that a specific recruitment step must commit Upf1p to the process and Upf1p-associated mRNAs to NMD.
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