ArticleMolecular and cellular probes2012
Dual probe DNA capture for sensitive real-time PCR detection of Cryptosporidium and Giardia.
Article in Molecular and cellular probes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Performance validation of the BD MAX Enteric Parasite Panel using simulated samples in low endemic regions.Parasites, hosts and diseases · 2025Article
- Detection and molecular characterization of major enteric pathogens in calves in central Ethiopia.BMC veterinary research · 2024Article
- Overview onJournal of tropical medicine · 2022Article
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting.The Korean journal of parasitology · 2016Article
- A Proposed Target Product Profile and Developmental Cascade for New Cryptosporidiosis Treatments.PLoS neglected tropical diseases · 2015Article
- Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples.The American journal of tropical medicine and hygiene · 2015Article
- A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium.The Lancet. Infectious diseases · 2015Review
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- The impoverished gut--a triple burden of diarrhoea, stunting and chronic disease.Nature reviews. Gastroenterology & hepatology · 2013Review
- Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Nucleic acid amplification for the enteropathogens Cryptosporidium and Giardia is complicated by low target template concentrations and PCR inhibitors. In this work we designed dual capture oligonucleotides for both Cryptosporidium and Giardia 18S rRNA targets which when utilized during DNA extraction from stool improved the limit of detection of our multiplex PCR assay by 1-2 logs, to as little as 10 cysts. When applied to clinical specimens, the method improved the real-time PCR C(T) by an average of 10.7 ± 9.7 cycles. This work provides a highly sensitive protocol for Cryptosporidium and Giardia when limit of detection is of utmost importance.
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Registered trials
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