ArticleApplied and environmental microbiology2012
High-resolution two-locus clonal typing of extraintestinal pathogenic Escherichia coli.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 2 of them syntheses that pooled it.
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Who cites it
115 citing papers in PubMed, 2 syntheses or guidelines pooled it, 180 citations in OpenAlex.
- Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages.Clinical microbiology reviews · 2019Pooled it
- Epidemic potential of Escherichia coli ST131 and Klebsiella pneumoniae ST258: a systematic review and meta-analysis.BMJ open · 2016Pooled it
- Characterisation of Haemolytic Uropathogenic Escherichia coli Isolated From Dogs and Cats.Veterinary medicine and science · 2026Article
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- Prevalence and characterization of ESBL-producing diarrheagenicFrontiers in microbiology · 2025Article
- Molecular epidemiology of a carbapenem-resistantFrontiers in microbiology · 2025Article
- Population structure and antibiotic resistance of swine extraintestinal pathogenic Escherichia coli from China.Nature communications · 2024Article
- Gut residentmedRxiv : the preprint server for health sciences · 2024Article
- Repeated Occurrence of Mobile Colistin Resistance Gene-Carrying Plasmids in PathogenicMicroorganisms · 2024Article
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- Genomic characterization of a multidrug-resistant uropathogenicAIMS microbiology · 2024Article
- Fluoroquinolone resistance in complicated urinary tract infections: association with the increased occurrence and diversity ofFrontiers in cellular and infection microbiology · 2024Article
- Clonal and resistance profiles of fluoroquinolone-resistant uropathogenicFrontiers in microbiology · 2024Article
- ArebioRxiv : the preprint server for biology · 2023Article
- Article
55 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
Abstract
Multilocus sequence typing (MLST) is usually based on the sequencing of 5 to 8 housekeeping loci in the bacterial chromosome and has provided detailed descriptions of the population structure of bacterial species important to human health. However, even strains with identical MLST profiles (known as sequence types or STs) may possess distinct genotypes, which enable different eco- or pathotypic lifestyles. Here we describe a two-locus, sequence-based typing scheme for Escherichia coli that utilizes a 489-nucleotide (nt) internal fragment of fimH (encoding the type 1 fimbrial adhesin) and the 469-nt internal fumC fragment used in standard MLST. Based on sequence typing of 191 model commensal and pathogenic isolates plus 853 freshly isolated clinical E. coli strains, this 2-locus approach-which we call CH (fumC/fimH) typing-consistently yielded more haplotypes than standard 7-locus MLST, splitting large STs into multiple clonal subgroups and often distinguishing different within-ST eco- and pathotypes. Furthermore, specific CH profiles corresponded to specific STs, or ST complexes, with 95% accuracy, allowing excellent prediction of MLST-based profiles. Thus, 2-locus CH typing provides a genotyping tool for molecular epidemiology analysis that is more economical than standard 7-locus MLST but has superior clonal discrimination power and, at the same time, corresponds closely to MLST-based clonal groupings.
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