ArticleJournal of immunological methods2012
A rapid and quantitative method for the evaluation of V gene usage, specificities and the clonal size of B cell repertoires.
Article in Journal of immunological methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- The immunodominant antibody response to Zika virus NS1 protein is characterized by cross-reactivity to self.The Journal of experimental medicine · 2021Article
- Microbial symbionts regulate the primary Ig repertoire.The Journal of experimental medicine · 2018Article
- Article
- The link between antibodies to OxLDL and natural protection against pneumococci depends on D(H) gene conservation.The Journal of experimental medicine · 2013Article
- Immunoglobulin analysis tool: a novel tool for the analysis of human and mouse heavy and light chain transcripts.Frontiers in immunology · 2012Article
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Authors and funding
10 authors.
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Abstract
The quantitative simultaneous description of both variable region gene usage and antigen specificity of immunoglobulin repertoires is a major goal in immunology. Current quantitative assays are labor intensive and depend on extensive gene expression cloning prior to screening for antigen specificity. Here we describe an alternative method based on high efficiency single B cell cultures coupled with RT-PCR that can be used for rapid characterization of immunoglobulin gene segment usage, clonal size and antigen specificity. This simplified approach should facilitate the study of antibody repertoires expressed by defined B cell subpopulations, the analysis of immune responses to self and nonself-antigens, the development and screening of synthetic antibodies and the accelerated study and screening of neutralizing antibodies to pathogenic threats.
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