ArticleBiophysical journal2012
Distribution of sialic acids on mucins and gels: a defense mechanism.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 69 citations in OpenAlex.
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- Mucus-derived glycans are inhibitory signals for Salmonella Typhimurium SPI-1-mediated invasion.Cell reports · 2025Article
- Development of a targeted oral pharmacologic duodenal exclusion therapy for the treatment of metabolic diseases.Science advances · 2025Article
- Mucus Physically Restricts Influenza A Viral Particle Access to the Epithelium.Advanced biology · 2025Article
- Herbal Medicine in Acute and Chronic Sinusitis; Still a Cinderella?Current allergy and asthma reports · 2025Review
- Reduced sialylation of airway mucin impairs mucus transport by altering the biophysical properties of mucin.Scientific reports · 2024Article
- Network and structural analysis of quail mucins with expression pattern of mucin 1 and mucin 4 in the intestines of the Iraqi common quail (Veterinary world · 2024Article
- Reduced Sialylation of Airway Mucin Impairs Mucus Transport by Altering the Biophysical Properties of Mucin.Research square · 2024Article
- Emergence of saliva protein genes in the secretory calcium-binding phosphoprotein (SCPP) locus and accelerated evolution in primates.bioRxiv : the preprint server for biology · 2024Article
- miR-125a-5p regulates the sialyltransferase ST3GAL1 in murine model of human intestinal campylobacteriosis.Gut pathogens · 2023Article
- Mucus increases cell iron uptake to impact the release of pro-inflammatory mediators after particle exposure.Scientific reports · 2023Article
- Review
- Capturing the start point of the virus-cell interaction with high-speed 3D single-virus tracking.Nature methods · 2022Article
- Mucosomes: Intrinsically Mucoadhesive Glycosylated Mucin Nanoparticles as Multi-Drug Delivery Platform.Advanced healthcare materials · 2022Article
- Adsorption of SARS CoV-2 spike proteins on various functionalized surfaces correlates with the high transmissibility of Delta and Omicron variants.Materials today. Bio · 2022Article
- Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism.Nature communications · 2021Article
- Thiolated Chitosans: A Multi-talented Class of Polymers for Various Applications.Biomacromolecules · 2021Review
- Multi-wavelength analytical ultracentrifugation as a tool to characterise protein-DNA interactions in solution.European biophysics journal : EBJ · 2020Article
- Changes in dietary fiber intake in mice reveal associations between colonic mucinGut microbes · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Moist mucosal epithelial interfaces that are exposed to external environments are dominated by sugar epitopes, some of which (e.g., sialic acids) are involved in host defense. In this study, we determined the abundance and distribution of two sialic acids to assess differences in their availability to an exogenous probe in isolated mucins and mucous gels. We used atomic force microscopy to obtain force maps of human preocular mucous and purified ocular mucins by probing and locating the interactions between tip-tethered lectins Maackia amurensis and Sambucus nigra and their respective receptors, α-2,3 and α-2,6 N-acetylneuraminic (sialic) acids. The rupture force distributions were not affected by neighboring sugar-bearing molecules. Energy contours for both lectin-sugar bonds were fitted to a two-barrier model, suggesting a conformational change before dissociation. In contrast to data from purified mucin molecules, the preocular gels presented numerous large clusters (19,000 ± 4000 nm(2)) of α-2,6 sialic acids, but very few small clusters (2000 ± 500 nm(2)) of α-2,3 epitopes. This indicates that mucins, which are rich in α-2,3 sialic acids, are only partially exposed at the surface of the mucous gel. Microorganisms that recognize α-2,3 sialic acids will encounter only isolated ligands, and the adhesion of other microorganisms will be enhanced by large islands of neighboring α-2,6 sialic acids. We have unveiled an additional level of mucosal surface heterogeneity, specifically in the distribution of pro- and antiadhesive sialic acids that protect underlying epithelia from viruses and bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.