ArticleBiophysical journal2012
Protein translocation through Tom40: kinetics of peptide release.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- The TOM Complex in the Outer Membrane of Mitochondria: A Supramolecular Assembly for Protein Import.Advances in experimental medicine and biology · 2026Review
- New insights into the structure and dynamics of the TOM complex in mitochondria.Biochemical Society transactions · 2024Review
- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Label-Free Detection of Post-translational Modifications with a Nanopore.Nano letters · 2019Article
- The Utility of Nanopore Technology for Protein and Peptide Sensing.Proteomics · 2018Review
- Paving the way to single-molecule protein sequencing.Nature nanotechnology · 2018Review
- Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore.Nature communications · 2018Article
- Article
- Peptide translocation across MOMP, the major outer membrane channel fromBiochemistry and biophysics reports · 2017Article
- Cation selectivity of the presequence translocase channel Tim23 is crucial for efficient protein import.eLife · 2017Article
- Role of Electroosmosis in the Permeation of Neutral Molecules: CymA and Cyclodextrin as an Example.Biophysical journal · 2016Article
- Reconstitution of the membrane protein OmpF into biomimetic block copolymer-phospholipid hybrid membranes.Beilstein journal of nanotechnology · 2016Article
- Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40.The Journal of biological chemistry · 2015Article
- Dynamics and Energy Contributions for Transport of Unfolded Pertactin through a Protein Nanopore.ACS nano · 2015Article
- Macromolecular mechanisms of protein translocation.Protein and peptide letters · 2014Review
- Watching single proteins using engineered nanopores.Protein and peptide letters · 2014Review
- Peptide translocation through the mesoscopic channel: binding kinetics at the single molecule level.European biophysics journal : EBJ · 2013Article
- Bacterial origin of a mitochondrial outer membrane protein translocase: new perspectives from comparative single channel electrophysiology.The Journal of biological chemistry · 2012Article
- Probing tubulin-blocked state of VDAC by varying membrane surface charge.Biophysical journal · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial proteins are almost exclusively imported into mitochondria from the cytosol in an unfolded or partially folded conformation. Regardless of whether they are destined for the outer or inner membrane, the intermembrane space, or the matrix, proteins begin the importation process by crossing the mitochondrial outer membrane via a specialized protein import machinery whose main component is the Tom40 channel. High-resolution ion conductance measurements through the Tom40 channel in the presence of the mitochondrial presequence peptide pF(1)β revealed the kinetics of peptide binding. Here we show that the rates for association k(on) and dissociation k(off) strongly depend on the applied transmembrane voltage. Both kinetic constants increase with an increase in the applied voltage. The increase of k(off) with voltage provides strong evidence of peptide translocation. This allows us to distinguish quantitatively between substrate blocking and permeation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.