ArticleResearch in pharmaceutical sciences2011
Optimization of the expression of reteplase in Escherichia coli.
Article in Research in pharmaceutical sciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Delaying production with prokaryotic inducible expression systems.Microbial cell factories · 2024Review
- Article
- Rational design of a new variant of Reteplase with optimized physicochemical profile and large-scale production in Escherichia coli.World journal of microbiology & biotechnology · 2022Article
- Reteplase: Structure, Function, and Production.Advanced biomedical research · 2019Review
- Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies.Research in pharmaceutical sciences · 2018Article
- Cloning and sequence analysis of a partial CDS of leptospiralVeterinary world · 2018Article
- High level expression of recombinant human growth hormone inResearch in pharmaceutical sciences · 2017Article
- Expression of a novel chimeric-truncated tPA in Pichia pastoris with improved biochemical properties.Molecular biotechnology · 2014Article
- Molecular cloning of Reteplase and its expression in E. coli using tac promoter.Advanced biomedical research · 2014Article
- Optimized condition for enhanced soluble-expression of recombinant mutant anabaena variabilis phenylalanine ammonia lyase.Advanced pharmaceutical bulletin · 2014Article
- Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a.Microbial cell factories · 2013Article
- Construction of an expression vector for production and purification of human somatostatin in Escherichia coli.Molecular biotechnology · 2013Article
- Cloning and Expression of Functional Reteplase in Escherichia coli TOP10.Avicenna journal of medical biotechnology · 2013Article
- Expression of the recombinant plasminogen activator (reteplase) by a non-lytic insect cell expression system.Research in pharmaceutical sciences · 2013Article
- Optimization of a single-chain antibody fragment overexpression in Escherichia coli using response surface methodology.Research in pharmaceutical sciencesArticle
- Optimization of Fermentation Conditions for Reteplase Expression byAvicenna journal of medical biotechnologyArticle
- Molecualr Cloning of the capsular antigen F1 of Yersinia pestis in pBAD/gIII plasmid.Research in pharmaceutical sciencesArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reteplase is a segment of tissue plasminogen activator used for the removal of thrombi in blood vessels. In the present study the cloned reteplase gene was used for its expression in competent E. coli. The recombinant plasmid, pET15b/reteplase (rpET-BL21), was transformed into competent E. coli strain BL21 (DE3) cells. Overnight culture of the transformed bacteria was induced by the addition of isopropylthio-ß-Dgalactoside (IPTG) to the final concentrations of 0.25, 0.5, 1 and 1.5 mM. Also, the effects of different temperatures(25, 30, 37 and 39°C), shaking speeds (100, 170 and 190 rpm), and various glucose concentrations (0.25, 0.5, 0.75 and 1 mM) on the expression of reteplase were examined. Samples were analyzed by SDS-PAGE. Maximum amount of protein production was obtained by the addition of 1 mM IPTG at 37°C, 100 rpm of shaking speed in the absence of glucose.
Indexed as
Identifiers
22224091PMC3249778W1581148528What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.