ArticleJournal of experimental botany2012
RBCS1A and RBCS3B, two major members within the Arabidopsis RBCS multigene family, function to yield sufficient Rubisco content for leaf photosynthetic capacity.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 113 citations in OpenAlex.
- Transfer of chloroplastSynthetic and systems biotechnology · 2027Article
- Review
- Chloroplast genome editing of Rubisco boosts photosynthesis and plant growth.Nature communications · 2026Article
- Exploring wild rices for photosynthetic efficiency improvement in rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2026Article
- Development of a High-Efficiency Hairy Root Transformation System for Diverse Cowpea (Plants (Basel, Switzerland) · 2026Article
- CC-Type GlutaredoxinPlants (Basel, Switzerland) · 2026Article
- Article
- Metabolomic and Single-Cell Transcriptomic Analyses Shed Light on Secondary Metabolite Profiling and Potential Developmental Dynamics of Glandular Trichomes in Artemisia argyi.Plant biotechnology journal · 2026Article
- Arbuscular Mycorrhizal Fungi-Mediated Reconfiguration of Poplar Leaf C-N-P Metabolic Networks: Environment-Dependent Synergies and Nutrient Interactions.Journal of fungi (Basel, Switzerland) · 2026Article
- Strategies to improve photosynthesis by modifying the RuBisCO system and its limitations.Molecular biology reports · 2025Review
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Bestrophin-like protein 4 is involved in photosynthetic acclimation to light fluctuations in Chlamydomonas.Plant physiology · 2024Article
- Knockdown ofPlants (Basel, Switzerland) · 2024Article
- Genome-Wide Analysis and Expression Profiling of SoybeanInternational journal of molecular sciences · 2024Article
- Metabolome and Transcriptome Association Analysis Reveals Mechanism of Synthesis of Nutrient Composition in Quinoa (Foods (Basel, Switzerland) · 2024Article
- Physiology, genomics, and evolutionary aspects of desert plants.Journal of advanced research · 2024Review
- Modulation of the wheat transcriptome by TaZFP13D under well-watered and drought conditions.Plant molecular biology · 2024Article
- An ancient metabolite damage-repair system sustains photosynthesis in plants.Nature communications · 2023Article
- Activity of Arabidopsis Rubisco small subunit promoter in various tissues of chickpea.3 Biotech · 2023Article
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (RBCS) is encoded by a nuclear RBCS multigene family in many plant species. The contribution of the RBCS multigenes to accumulation of Rubisco holoenzyme and photosynthetic characteristics remains unclear. T-DNA insertion mutants of RBCS1A (rbcs1a-1) and RBCS3B (rbcs3b-1) were isolated among the four Arabidopsis RBCS genes, and a double mutant (rbcs1a3b-1) was generated. RBCS1A mRNA was not detected in rbcs1a-1 and rbcs1a3b-1, while the RBCS3B mRNA level was suppressed to ∼20% of the wild-type level in rbcs3b-1 and rbcs1a3b-1 leaves. As a result, total RBCS mRNA levels declined to 52, 79, and 23% of the wild-type level in rbcs1a-1, rbcs3b-1, and rbcs1a3b-1, respectively. Rubisco contents showed declines similar to total RBCS mRNA levels, and the ratio of Rubisco-nitrogen to total nitrogen was 62, 78, and 40% of the wild-type level in rbcs1a-1, rbcs3b-1, and rbcs1a3b-1, respectively. The effects of RBCS1A and RBCS3B mutations in rbcs1a3b-1 were clearly additive. The rates of CO(2) assimilation at ambient CO(2) of 40 Pa were reduced with decreased Rubisco contents in the respective mutant leaves. Although the RBCS composition in the Rubisco holoenzyme changed, the CO(2) assimilation rates per unit of Rubisco content were the same irrespective of the genotype. These results clearly indicate that RBCS1A and RBCS3B contribute to accumulation of Rubisco in Arabidopsis leaves and that these genes work additively to yield sufficient Rubisco for photosynthetic capacity. It is also suggested that the RBCS composition in the Rubisco holoenzyme does not affect photosynthesis under the present ambient [CO(2)] conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.