ArticleElectrophoresis2012
Bleach gel: a simple agarose gel for analyzing RNA quality.
Article in Electrophoresis, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 298 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
298 citing papers in PubMed.
- Influence of the administration route and dose on the expression and antibody responses of a reporter and avian influenza self-amplifying mRNA vaccine in poultry.The veterinary quarterly · 2026Article
- Intervertebral disc degeneration is accelerated by GH overexpression but attenuated by GH antagonism.GeroScience · 2026Article
- Insights into the genetic bases of differential organ size in cactus flowers: a case study in two contrasting species.Annals of botany · 2026Article
- Multi-Omics and Functional Analysis ofToxins · 2026Article
- MtvS1 and MtvS2 Interact with RNA polymerase to regulate the Francisella type V-A CRISPR-Cas system.Nature communications · 2026Article
- TagR, a newly identified member of the MarR family of transcriptional regulators, represses the NRPS operon inMicrobiology spectrum · 2026Article
- Nucleoside-Modified mRNA Encoding Alpha-Galactosidase A Ameliorates Fabry Disease Phenotypes in Human IPSC-Derived Cardiomyocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Impact of dietary myo-inositol supplements on immune cell distribution and cytokine expression in laying hens of two different strains.Poultry science · 2026Article
- Total fungal RNA extraction from complex solid- state fermentation products.Applied microbiology and biotechnology · 2026Article
- Metagenomics and metatranscriptomics of prokaryotic and fungal microbiomes in produced water associated with petroleum degradation and pipeline corrosion from an oil terminal in Brazil.World journal of microbiology & biotechnology · 2026Article
- Impact of feeding diets without mineral P supplement on the immune system of two laying hen strains.Poultry science · 2026Article
- Sexual aggression and prenatal stress lead to poor maternal care and aggressive behaviour in male Wistar rat offspring.IBRO neuroscience reports · 2026Article
- The Quorum-Sensing Regulator SdiA ActivatesMicroorganisms · 2026Article
- Gene expression plasticity under multiple stresses drives higher tolerance to a macrolide in saline and warmer environments.npj antimicrobials and resistance · 2026Article
- Optimized Protocols to Extract Total Transcripts and Proteins from Lipid-Rich Tissues.Methods and protocols · 2026Article
- Effects of dietary myo-inositol supplementation on the expression of fibroblast growth factor 23 (FGF23) and αKlotho in two commercial laying hen strains.Poultry science · 2026Article
- Efficient genetic perturbation of murine sensory neurons in vivo using CRISPR/Cas9.The journal of pain · 2026Article
- Transcriptomic and epigenetic alterations in aging-related genes in the medaka liver due to ancestral BPA exposure.Journal of the Endocrine Society · 2026Article
- The Rana sylvatica skin-secreted antimicrobial peptide (AMP) gene repertoire highlights broader patterns in anuran AMP evolution.Scientific reports · 2026Article
- Comprehensive high-throughput sequence-based detection and RT-LAMP confirmation of RNA viruses associated with dragon fruit (Selenicereus spp.) in India.Archives of virology · 2026Article
238 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive equipment such as microfluidic electrophoretic devices, and as an alternative to the costly and time-consuming standard formaldehyde gel, RNA quality can be quickly analyzed by adding small amounts of commercial bleach to TAE buffer-based agarose gels prior to electrophoresis. In the presence of low concentrations of bleach, the secondary structure of RNA is denatured and potential contaminating RNases are destroyed. Because of this, the 'bleach gel' is a functional approach that addresses the need for an inexpensive and safe way to evaluate RNA integrity and will improve the ability of researchers to rapidly analyze RNA quality.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.