ArticleNature protocols2012
Tracking mechanics and volume of globular cells with atomic force microscopy using a constant-height clamp.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 55 citations in OpenAlex.
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- In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in the membrane for uniaxial substrate stretch.Medical & biological engineering & computing · 2021Article
- Mapping mechanical properties of biological materials via an add-on Brillouin module to confocal microscopes.Nature protocols · 2021Article
- The nucleus acts as a ruler tailoring cell responses to spatial constraints.Science (New York, N.Y.) · 2020Article
- Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy.Biology open · 2020Article
- F-Actin Interactome Reveals Vimentin as a Key Regulator of Actin Organization and Cell Mechanics in Mitosis.Developmental cell · 2020Article
- The Mechanics of Mitotic Cell Rounding.Frontiers in cell and developmental biology · 2020Review
- Cdk1-mediated DIAPH1 phosphorylation maintains metaphase cortical tension and inactivates the spindle assembly checkpoint at anaphase.Nature communications · 2019Article
- Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding.Nature communications · 2017Article
- Actin cortex architecture regulates cell surface tension.Nature cell biology · 2017Article
- Multi-scale computational study of the mechanical regulation of cell mitotic rounding in epithelia.PLoS computational biology · 2017Article
- Effect of dacarbazine on CD44 in live melanoma cells as measured by atomic force microscopy-based nanoscopy.International journal of nanomedicine · 2017Article
- Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling.Scientific reports · 2016Article
- Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement.Nature communications · 2015Article
- Mechanical control of mitotic progression in single animal cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Investigating cell mechanics with atomic force microscopy.Journal of the Royal Society, Interface · 2015Article
- Cdk1-dependent mitotic enrichment of cortical myosin II promotes cell rounding against confinement.Nature cell biology · 2015Article
- Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis.The EMBO journal · 2015Article
- Measurement science in the circulatory system.Cellular and molecular bioengineering · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the role of physical forces at a cellular level, it is necessary to track mechanical properties during cellular processes. Here we present a protocol that uses flat atomic force microscopy (AFM) cantilevers clamped at constant height, and light microscopy to measure the resistance force, mechanical stress and volume of globular animal cells under compression. We describe the AFM and cantilever setup, live cell culture in the AFM, how to ensure stability of AFM measurements during medium perfusion, integration of optical microscopy to measure parameters such as volume and track intracellular dynamics, and interpretation of the physical parameters measured. Although we use this protocol on trypsinized interphase and mitotic HeLa cells, it can also be applied to other cells with a relatively globular shape, especially animal cells in a low-adhesive environment. After a short setup phase, the protocol can be used to investigate approximately one cell per hour.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.