ArticleMethods in molecular biology (Clifton, N.J.)2012
Morpholino injection in Xenopus.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Tissue-specific in vivo transformation of plasmid DNA in Neotropical tadpoles using electroporation.PloS one · 2023Article
- The Many Faces ofFrontiers in physiology · 2019Article
- Evolutionarily conservedProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- A Matter of the Heart: The African Clawed Frog Xenopus as a Model for Studying Vertebrate Cardiogenesis and Congenital Heart Defects.Journal of cardiovascular development and disease · 2016Review
- The Lhx9-integrin pathway is essential for positioning of the proepicardial organ.Development (Cambridge, England) · 2016Article
- Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation.Genetics · 2016Article
- Budgett's frog (Lepidobatrachus laevis): A new amphibian embryo for developmental biology.Developmental biology · 2015Article
- Article
- Congenital heart disease protein 5 associates with CASZ1 to maintain myocardial tissue integrity.Development (Cambridge, England) · 2014Article
- RNA-seq in the tetraploid Xenopus laevis enables genome-wide insight in a classic developmental biology model organism.Methods (San Diego, Calif.) · 2014Article
- Facial transplants in Xenopus laevis embryos.Journal of visualized experiments : JoVE · 2014Article
- Proteomic profiling of cardiac tissue by isolation of nuclei tagged in specific cell types (INTACT).Development (Cambridge, England) · 2014Article
- Tcf21 regulates the specification and maturation of proepicardial cells.Development (Cambridge, England) · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The study of gene function in developmental biology has been significantly furthered by advances in antisense technology made in the early 2000s. This was achieved, in particular, by the introduction of morpholino (MO) oligonucleotides. The introduction of antisense MO oligonucleotides into cells enables researchers to readily reduce the levels of their protein of interest without investing huge financial or temporal resources, in both in vivo and in vitro model systems. Historically, the African clawed frog Xenopus has been used to study vertebrate embryological development, due to its ability to produce vast numbers of offspring that develop rapidly, in synchrony, and can be cultured in buffers with ease. The developmental progress of Xenopus embryos has been extensively characterized and this model organism is very easy to maintain. It is these attributes that enable MO-based knockdown strategies to be so effective in Xenopus. In this chapter, we will detail the methods of microinjecting MO oligonucleotides into early embryos of X. laevis and X. tropicalis. We will discuss how MOs can be used to prevent either pre-mRNA splicing or translation of the specific gene of interest resulting in abrogation of that gene's function and advise on what control experiments should be undertaken to verify their efficacy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.