ArticleMicrobiology (Reading, England)2012
Pathogenic Naegleria fowleri and non-pathogenic Naegleria lovaniensis exhibit differential adhesion to, and invasion of, extracellular matrix proteins.
Article in Microbiology (Reading, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
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- Reduced cell-substrate adhesion formation promotes cell migration inbioRxiv : the preprint server for biology · 2025Article
- Eating the brain - A multidisciplinary study provides new insights into the mechanisms underlying the cytopathogenicity of Naegleria fowleri.PLoS pathogens · 2025Article
- Noninvasive diagnostic biomarkers, genomic profiling, and advanced microscopic imaging in the early detection and characterization ofAnnals of medicine and surgery (2012) · 2024Review
- pH-regulated single cell migration.Pflugers Archiv : European journal of physiology · 2024Review
- Detection and Confirmation ofJournal of parasitology research · 2024Article
- Transcriptomic profiling of "brain-eating amoeba"Frontiers in cellular and infection microbiology · 2024Article
- Utilizing metagenomic next-generation sequencing and phylogenetic analysis to identify a rare pediatric case ofFrontiers in microbiology · 2024Article
- Characterization of the extracellular vesicles, ultrastructural morphology, and intercellular interactions of multiple clinical isolates of the brain-eating amoeba,Frontiers in microbiology · 2023Article
- Higher amoebic and metronidazole resistant forms of Blastocystis sp. seen in schizophrenic patients.Parasites & vectors · 2022Article
- Article
- Various brain-eating amoebae: the protozoa, the pathogenesis, and the disease.Frontiers of medicine · 2021Review
- Naphthyridine Derivatives Induce Programmed Cell Death inPharmaceuticals (Basel, Switzerland) · 2021Article
- Primary Amoebic Meningoencephalitis byBiomolecules · 2021Review
- Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri.BMC biology · 2021Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- Isolation and Identification ofPathogens (Basel, Switzerland) · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Naegleria fowleri and Naegleria lovaniensis are closely related free-living amoebae found in the environment. N. fowleri causes primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system, while N. lovaniensis is non-pathogenic. N. fowleri infection occurs when the amoebae access the nasal passages, attach to the nasal mucosa and its epithelial lining, and migrate to the brain. This process involves interaction with components of the host extracellular matrix (ECM). Since the ability to invade tissues can be a characteristic that distinguishes pathogenic from non-pathogenic amoebae, the objective of this study was to assess adhesion to, and invasion of, the ECM by these two related but distinct Naegleria species. N. fowleri exhibited a higher level of adhesion to the ECM components laminin-1, fibronectin and collagen I. Scanning electron microscopy revealed that N. fowleri attached on ECM substrata exhibited a spread-out appearance that included the presence of focal adhesion-like structures. Western immunoblotting revealed two integrin-like proteins for both species, but one of these, with a molecular mass of approximately 70 kDa, was detected at a higher level in N. fowleri. Confocal microscopy indicated that the integrin-like proteins co-localized to the focal adhesion-like structures. Furthermore, anti-integrin antibody decreased adhesion of N. fowleri to ECM components. Finally, N. fowleri disrupted 3D ECM scaffolds, while N. lovaniensis had a minimal effect. Collectively, these results indicate a distinction in adhesion to, and invasion of, ECM proteins between N. fowleri and N. lovaniensis.
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Registered trials
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