ArticleThe Journal of surgical research2012
Human isolates of Cronobacter sakazakii bind efficiently to intestinal epithelial cells in vitro to induce monolayer permeability and apoptosis.
Article in The Journal of surgical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- The Role ofFoods (Basel, Switzerland) · 2022Article
- Necrotizing Enterocolitis: Overview on In Vitro Models.International journal of molecular sciences · 2021Review
- Current and Future Perspectives on the Role of Probiotics, Prebiotics, and Synbiotics in Controlling PathogenicFrontiers in microbiology · 2021Review
- Article
- PNU-282987 Attenuates Intestinal Epithelial Barrier Dysfunction in LPS-Induced Endotoxemia.Inflammation · 2020Article
- Bacteroides fragilis Strain ZY-312 Defense against Cronobacter sakazakii-Induced Necrotizing EnterocolitismSystems · 2019Article
- Effects of artificially introduced Enterococcus faecalis strains in experimental necrotizing enterocolitis.PloS one · 2019Article
- Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis.PloS one · 2017Article
- Review
- Roles of nitric oxide and intestinal microbiota in the pathogenesis of necrotizing enterocolitis.Journal of pediatric surgery · 2016Review
- Pathogenesis of neonatal necrotizing enterocolitis.Pediatric surgery international · 2015Review
- A Cronobacter turicensis O1 antigen-specific monoclonal antibody inhibits bacterial motility and entry into epithelial cells.Infection and immunity · 2015Article
- The Membrane Proteins Involved in Virulence of Cronobacter sakazakii Virulent G362 and Attenuated L3101 Isolates.Frontiers in microbiology · 2015Article
- Adherence inhibition of Cronobacter sakazakii to intestinal epithelial cells by lactoferrin.Current microbiology · 2014Article
- Cronobacter: an emergent pathogen causing meningitis to neonates through their feeds.Science progress · 2014Article
- Cronobacter: an emerging opportunistic pathogen associated with neonatal meningitis, sepsis and necrotizing enterocolitis.Journal of perinatology : official journal of the California Perinatal Association · 2013Review
- The role of the intestinal microbiota in the pathogenesis of necrotizing enterocolitis.Seminars in pediatric surgery · 2013Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundCronobacter sakazakii (CS) is an emerging opportunistic pathogen that causes life-threatening infections in infants. This pathogen has been implicated in the outbreaks of necrotizing enterocolitis (NEC) with associated rates of high mortality and morbidity. In this study, we compared the abilities of CS strains isolated from human and environmental sources to bind to intestinal epithelial cells and trigger apoptosis. MATERIALS AND
methodsCS strains were isolated from human and environmental sources and their abilities to bind to intestinal epithelial cells were determined. Monolayer permeability was determined by transepithelial electrical resistance (TEER) and horseradish peroxidase (HRP) leakage. Apoptosis was examined by ApoTag and AnnexinV-7AAD staining. PKC activation was evaluated by non-radioactive PepTag assay.
resultsHuman isolates of CS bind to rat and human enterocytes more efficiently than environmental strains. Additionally, these strains induced increased enterocyte monolayer permeability as indicated by a decrease in TEER and an increase in transcellular leakage of exogenously added HRP. Human isolates also caused tight junction disruption and significant apoptosis of enterocytes compared with environmental strains due to increased production of inducible nitric oxide. We also observed that human CS isolates caused 2-fold increase in the activation of phosphokinase C (PKC) than environmental strains. Blocking the PKC activity in enterocytes by an inhibitor, Gö 6983, suppressed CS-mediated tight junction disruption, monolayer permeability, and apoptosis of the cells.
conclusionThese results suggest that human isolates of CS more efficiently bind to and cause damage to intestinal epithelial cells compared with environmental strains.
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