ArticleMolecular microbiology2012
The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 84 citations in OpenAlex.
- The RpfF Acyl-ACP thioesterase of major rice pathogen Xanthomonas oryzae pv. oryzae is essential for maintenance of membrane function and virulence.PLoS pathogens · 2026Article
- A recombinant lysogenic bacteriophage inhibitsAntimicrobial agents and chemotherapy · 2026Article
- Quorum sensing in theApplied and environmental microbiology · 2026Review
- Functional disparities of malonyl-ACP decarboxylase betweenApplied and environmental microbiology · 2025Article
- BDSF Analogues Inhibit Quorum Sensing-Regulated Biofilm Production inMicroorganisms · 2024Article
- A FadR-Type Regulator Activates the Biodegradation of Polycyclic Aromatic Hydrocarbons by Mediating Quorum Sensing in Croceicoccus naphthovorans Strain PQ-2.Applied and environmental microbiology · 2023Article
- The BDSF quorum sensing receptor RpfR regulates Bep exopolysaccharide synthesis in Burkholderia cenocepacia via interaction with the transcriptional regulator BerB.NPJ biofilms and microbiomes · 2022Article
- TheApplied and environmental microbiology · 2022Review
- The diffusible signal factor synthase, RpfF, in Xanthomonas oryzae pv. oryzae is required for the maintenance of membrane integrity and virulence.Molecular plant pathology · 2022Article
- Diffusible Signal Factors Act through AraC-Type Transcriptional Regulators as Chemical Cues To Repress Virulence of Enteric Pathogens.Infection and immunity · 2020Article
- One gene, multiple ecological strategies: A biofilm regulator is a capacitor for sustainable diversity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Two Functional Fatty Acyl Coenzyme A Ligases Affect Free Fatty Acid Metabolism To Block Biosynthesis of an Antifungal Antibiotic in Lysobacter enzymogenes.Applied and environmental microbiology · 2020Article
- AFrontiers in microbiology · 2020Article
- A novel 3-oxoacyl-ACP reductase (FabG3) is involved in the xanthomonadin biosynthesis of Xanthomonas campestris pv. campestris.Molecular plant pathology · 2019Article
- Versatile biomanufacturing through stimulus-responsive cell-material feedback.Nature chemical biology · 2019Article
- Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF.PLoS biology · 2019Article
- Article
- Quorum Sensing Signaling and Quenching in the Multidrug-Resistant PathogenFrontiers in cellular and infection microbiology · 2018Review
- Mutation of the cyclic di-GMP phosphodiesterase gene in Burkholderia lata SK875 attenuates virulence and enhances biofilm formation.Journal of microbiology (Seoul, Korea) · 2017Article
- Reclassification of the Specialized Metabolite Producer Pseudomonas mesoacidophila ATCC 31433 as a Member of the Burkholderia cepacia Complex.Journal of bacteriology · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Signal molecules of the diffusible signal factor (DSF) family have been shown recently to be involved in regulation of pathogenesis and biofilm formation in diverse Gram-negative bacteria. DSF signals are reported to be active not only on their cognate bacteria, but also on unrelated bacteria and the pathogenic yeast, Candida albicans. DSFs are monounsaturated fatty acids of medium chain length containing an unusual cis-2 double bond. Although genetic analyses had identified genes involved in DSF synthesis, the pathway of DSF synthesis was unknown. The DSF of the important human pathogen Burkholderia cenocepacia (called BDSF) is cis-2-dodecenoic acid. We report that BDSF is synthesized from a fatty acid synthetic intermediate, the acyl carrier protein (ACP) thioester of 3-hydroxydodecanoic acid. This intermediate is intercepted by protein Bcam0581 and converted to cis-2-dodecenoyl-ACP. Bcam0581 is annotated as a homologue of crotonase, the first enzyme of the fatty acid degradation pathway. We demonstrated Bcam0581to be a bifunctional protein that not only catalysed dehydration of 3-hydroxydodecanoyl-ACP to cis-2-dodecenoyl-ACP, but also cleaved the thioester bond to give the free acid. Both activities required the same set of active-site residues. Although dehydratase and thioesterase activities are known activities of the crotonase superfamily, Bcam0581 is the first protein shown to have both activities.
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