ArticleThe Biochemical journal2012
Catalytic residues and a predicted structure of tetrahydrobiopterin-dependent alkylglycerol mono-oxygenase.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Adaptations of the 3T3-L1 adipocyte lipidome to defective ether lipid catabolism upon Agmo knockdown.Journal of lipid research · 2022Article
- The Emerging Physiological Role of AGMO 10 Years after Its Gene Identification.Life (Basel, Switzerland) · 2021Review
- Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.Human genetics · 2019Article
- Alkylglycerol monooxygenase, a heterotaxy candidate gene, regulates left-right patterning via Wnt signaling.Developmental biology · 2019Article
- Structural Model of the ETR1 Ethylene Receptor Transmembrane Sensor Domain.Scientific reports · 2019Article
- Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.The Journal of infectious diseases · 2018Article
- Oxidative cyclization of prodigiosin by an alkylglycerol monooxygenase-like enzyme.Nature chemical biology · 2017Article
- Cuticle integrity and biogenic amine synthesis in Caenorhabditis elegans require the cofactor tetrahydrobiopterin (BH4).Genetics · 2015Article
- Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Alkylglycerol monooxygenase.IUBMB life · 2013Review
- Orphan enzymes in ether lipid metabolism.Biochimie · 2013Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Alkylglycerol mono-oxygenase (EC 1.14.16.5) forms a third, distinct, class among tetrahydrobiopterin-dependent enzymes in addition to aromatic amino acid hydroxylases and nitric oxide synthases. Its protein sequence contains the fatty acid hydroxylase motif, a signature indicative of a di-iron centre, which contains eight conserved histidine residues. Membrane enzymes containing this motif, including alkylglycerol mono-oxygenase, are especially labile and so far have not been purified to homogeneity in active form. To obtain a first insight into structure-function relationships of this enzyme, we performed site-directed mutagenesis of 26 selected amino acid residues and expressed wild-type and mutant proteins containing a C-terminal Myc tag together with fatty aldehyde dehydrogenase in Chinese-hamster ovary cells. Among all of the acidic residues within the eight-histidine motif, only mutation of Glu137 to alanine led to an 18-fold increase in the Michaelis-Menten constant for tetrahydrobiopterin, suggesting a role in tetrahydrobiopterin interaction. A ninth additional histidine residue essential for activity was also identified. Nine membrane domains were predicted by four programs: ESKW, TMHMM, MEMSAT and Phobius. Prediction of a part of the structure using the Rosetta membrane ab initio method led to a plausible suggestion for a structure of the catalytic site of alkylglycerol mono-oxygenase.
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