ArticleSensors (Basel, Switzerland)2010
Development of a quartz crystal microbalance biosensor with aptamers as bio-recognition element.
Article in Sensors (Basel, Switzerland), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 89 citations in OpenAlex.
- Review
- Antifouling hydrogel film based on a sandwich array for salivary glucose monitoring.RSC advances · 2021Article
- Recent Advances on Detection of Insecticides Using Optical Sensors.Sensors (Basel, Switzerland) · 2021Review
- Guide to Selecting a Biorecognition Element for Biosensors.Bioconjugate chemistry · 2018Review
- The Resistance-Amplitude-Frequency Effect of In-Liquid Quartz Crystal Microbalance.Sensors (Basel, Switzerland) · 2017Article
- Critical overview on the application of sensors and biosensors for clinical analysis.Trends in analytical chemistry : TRAC · 2016Review
- A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor.Biomicrofluidics · 2016Article
- A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.Sensors (Basel, Switzerland) · 2015Article
- Integration of biosensors and drug delivery technologies for early detection and chronic management of illness.Sensors (Basel, Switzerland) · 2013Review
- RNA plasticity and selectivity applicable to therapeutics and novel biosensor development.Genes to cells : devoted to molecular & cellular mechanisms · 2012Review
- Electrochemical sensors and biosensors.Analytical chemistry · 2012Review
- Graphene and other nanomaterial-based electrochemical aptasensors.Biosensors · 2012Review
- Novel platform development using an assembly of carbon nanotube, nanogold and immobilized RNA capture element towards rapid, selective sensing of bacteria.Sensors (Basel, Switzerland) · 2012Article
- Light assisted antibody immobilization for bio-sensing.Biomedical optics express · 2011Article
- Aptamer in bioanalytical applications.Analytical chemistry · 2011Review
- Advances in electronic-nose technologies developed for biomedical applications.Sensors (Basel, Switzerland) · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ultimate goal in any biosensor development project is its use for actual sample detection. Recently, there has been an interest in biosensors with aptamers as bio-recognition elements, but reported examples all deal with standards, not human serum. In order to verify the differences of aptamer-based biosensor and antibody-based biosensor in clinical detection, a comparison of the performance of aptamer-based and antibody-based quartz crystal microbalance (QCM) biosensors for the detection of immunoglobulin E (IgE) in human serum was carried out. Aptamers (or antibodies) specific to IgE were immobilized on the gold surface of a quartz crystal. The frequency shifts of the QCM were measured. The linear range with the antibody (10-240 μg/L) compared to that of the aptamer (2.5-200 μg/L), but a lower detection limit could be observed in the aptamer-based biosensor. The reproducibility of the two biosensors was comparable. The aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity. In addition, the aptamer receptors could tolerate repeated affine layer regeneration after ligand binding and recycling of the biosensor with little loss of sensitivity. When stored for three weeks, the frequency shifts of the aptamer-coated crystals were all greater than 90% of those on the response at the first day.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.