ArticleMolecular vision2011
Retro-orbital injection of FITC-dextran is an effective and economical method for observing mouse retinal vessels.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Imaging evidence of diabetic choroidopathy in vivo: angiographic pathoanatomy and choroidal-enhanced depth imaging.PloS one · 2013Trial
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- The gut microbiota regulates diabetic retinopathy in adult rats.Frontiers in microbiology · 2025Article
- Biodistribution of PET radiotracers in tumor-bearing TRAMP mice administered by retroorbital or jugular vein injections.Scientific reports · 2024Article
- Protective effects of nattokinase against microvasculopathy and neuroinflammation in diabetic retinopathy.Journal of diabetes · 2023Article
- Morphine pharmacokinetics and opioid transporter expression at the blood-retina barrier of male and female mice.Frontiers in pharmacology · 2023Article
- Role of the JAK/STAT pathway in a streptozotocin-induced diabetic retinopathy mouse model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2022Article
- Fast volumetric imaging with line-scan confocal microscopy by electrically tunable lens at resonant frequency.Optics express · 2022Article
- In vitro induction and intraocular application in oxygen-induced retinopathy of adipose-derived mesenchymal stem cells.Molecular vision · 2022Article
- Expression of the neuroprotective factors BDNF, CNTF, and FGF-2 in normal and oxygen induced retinopathy.Frontiers in neuroscience · 2022Article
- Ly6c as a New Marker of Mouse Blood Vessels: Qualitative and Quantitative Analyses on Intact and Ischemic Retinas.International journal of molecular sciences · 2021Article
- Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect.BMC ophthalmology · 2021Article
- Nattokinase Attenuates Retinal Neovascularization Via Modulation of Nrf2/HO-1 and Glial Activation.Investigative ophthalmology & visual science · 2021Article
- Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis.Theranostics · 2021Article
- The Binding of CD93 to Multimerin-2 Promotes Choroidal Neovascularization.Investigative ophthalmology & visual science · 2020Article
- Rb1/Rbl1/Vhl loss induces mouse subretinal angiomatous proliferation and hemangioblastoma.JCI insight · 2019Article
- Methods to label, image, and analyze the complex structural architectures of microvascular networks.Microcirculation (New York, N.Y. : 1994) · 2019Review
- microRNA-218 Inhibits Oxygen-induced Retinal Neovascularization via Reducing the Expression of Roundabout 1.Chinese medical journal · 2016Article
- Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus.Vascular cell · 2015Article
- Sulodexide inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy.BMB reports · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo assess the effects of retro-orbital (RO) injection of fluorescein isothiocyanate dextran (FITC-dextran) for observing mouse retinal vessels.
methodsOxygen-induced retinopathy (OIR) was induced in 7-day postnatal (P7) C57BL/6J mice by exposing them to a 75% oxygen atmosphere for 5 days and then returning them to room air at P12. At P17, 45 P17 OIR mice and 12 normal mice received an RO injection of FITC-dextran. Five P17 OIR mice were perfused with FITC-dextran via the left ventricle. Retinal flatmounts were viewed under a fluorescence microscope and a confocal microscope. Following RO injection or left ventricular (LV) perfusion, the areas of neovascularization, as well as the total retina areas, were measured and analyzed using Image Pro-Plus 5.1 software.
resultsThe suitable volume of FITC-dextran for RO injection to observe the retinal vessels of a P17 OIR mouse was 0.05 ml, the 5% volume required to achieve similar results by administering FITC-dextran using LV perfusion. The total retinal vessels in P17 OIR mice could be fully observed under a fluorescence microscope 3 s after RO injection of FITC-dextran. Our study showed that RO injection of FITC-dextran was suitable for observing the retinal vessels of all postnatal mice. There was no significant difference in evaluating retinal neovascularization between RO injection and LV perfusion of FITC-dextran (p>0.05).
conclusionsRetro-orbital injection of FITC-dextran is an economical method for observing retinal vessels in mice, when compared with LV perfusion of FITC-dextran.
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Identifiers
22219652PMC3250377W2148104186What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.