ArticleMolecular vision2011
Cornea lenticule viability and structural integrity after refractive lenticule extraction (ReLEx) and cryopreservation.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 2 syntheses or guidelines pooled it, 68 citations in OpenAlex.
- Efficacy and safety of small-incision corneal intrastromal lenticule implantation for hyperopia correction: a systematic review and meta-analysis.Frontiers in medicine · 2024Pooled it
- Femtosecond laser-assisted implantation of corneal stroma lenticule for keratoconus.International ophthalmology · 2021Pooled it
- Trial
- Preservation and Reuse of KLEx Lenticules: From Biobanking to Therapeutic and Refractive Innovations.Bioengineering (Basel, Switzerland) · 2026Review
- Ex Vivo Assessment of Excimer Laser-Assisted Lenticule Customization.Translational vision science & technology · 2026Article
- Biological assessment and preservation strategies for SMILE- derived lenticules: towards a corneal regenerative biobank.Scientific reports · 2026Article
- Biomechanical changes and associated factors of preserved SMILE-derived corneal stromal lenticules.Eye (London, England) · 2026Article
- The Preservation and Reuse of Lenticules Extracted via Small Incision Lenticule Extraction (SMILE): A Narrative Review.Bioengineering (Basel, Switzerland) · 2025Review
- Banking of post-SMILE stromal lenticules for additive keratoplasty: A new challenge for eye banks?International ophthalmology · 2024Review
- Ex Vivo Lenticule Customization for Stromal Lenticule Addition Keratoplasty.Investigative ophthalmology & visual science · 2024Article
- The new future perspective in corneal tissue utilisation - methods of preparation and preservation.BMC ophthalmology · 2023Article
- Update of Research Progress on Small Incision Lenticule Extraction (SMILE) Lenticule Reuse.Clinical ophthalmology (Auckland, N.Z.) · 2023Review
- Preservation of corneal stromal lenticule: review.Cell and tissue banking · 2022Review
- Corneal Donation: Current Guidelines and Future Direction.Clinical ophthalmology (Auckland, N.Z.) · 2021Review
- Banking of corneal stromal lenticules: a risk-analysis assessment with the EuroGTP II interactive tool.Cell and tissue banking · 2020Article
- Corneal remodelling and topography following biological inlay implantation with combined crosslinking in a rabbit model.Scientific reports · 2019Article
- Decellularized Human Stromal Lenticules Combine with Corneal Epithelial-Like Cells: A New Resource for Corneal Tissue Engineering.Stem cells international · 2019Article
- Corneal thickening and central flattening induced by femtosecond laser hyperopic-shaped intrastromal lenticule implantation.International ophthalmology · 2017Article
- Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts.Scientific reports · 2017Article
- Decellularization of human stromal refractive lenticules for corneal tissue engineering.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo assess and compare keratocyte viability and collagen structure in cornea stroma lenticules collected immediately after refractive lenticule extraction (ReLEx) and one month after cryopreservation.
methodsThe fresh and cryopreserved human stroma lenticules procured after ReLEx were processed for ultrastructural analysis of keratocytes and collagen fibrils with transmission electron microscopy (TEM), apoptotic cell detection with deoxynucleotidyl transferase-mediated nick end labeling assay (TUNEL) assay, and cultured for keratocyte-specific gene expression analysis using reverse transcriptase polymerase chain reaction (RT-PCR).
resultsThe periphery of the lenticule had greater TUNEL-positive cells compared to the center of the lenticule in both fresh and cryopreserved groups. There was an increase in TUNEL-positive cells after cryopreservation, which was significantly higher in the center of the lenticule, but not in the periphery. TEM showed apoptotic, necrotic and viable quiescent keratocytes in fresh and cryopreserved lenticules. Collagen analysis with TEM showed a well preserved and well aligned structure in fresh and cryopreserved lenticules; without significant change in the total number of collagen fibrils but with an increased collagen fibril density (CFD) after cryopreservation. In vitro, isolated keratocytes derived from fresh and cryopreserved lenticules exhibited a typical fibroblastic phenotype. RT-PCR showed a positive gene expression for keratocan (KERA) and aldehyde dehydrogenase 3A1 (ALDH3A1) in cells isolated from fresh and cryopreserved lenticules.
conclusionsThe stromal lenticules extracted from ReLEx surgery remain viable after cryopreservation. Although they showed a decrease in CFD, the collagen architecture was preserved and there was good cellular viability.
Indexed as
Identifiers
22219639PMC3249438W2156056883What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.