ArticleMolecular vision2011
Inhibition by female sex hormones of collagen degradation by corneal fibroblasts.
Article in Molecular vision, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Sex Hormone Imbalance in Keratoconus: Estrogen-Mediated Collagen Remodeling and Biomechanical Weakening.Investigative ophthalmology & visual science · 2026Article
- Sexual dimorphism in keratoconus: transcriptomic and hormonal mechanisms underlying stromal remodelling.Eye and vision (London, England) · 2026Article
- Multidimensional regulation of estrogen signaling in pelvic floor connective tissue homeostasis and remodeling.Frontiers in immunology · 2026Review
- Ovarian steroids modulate mRNA expression of ECM associated genes and collagen deposition induced by TGF β1 in equine endometrium in vitro.Scientific reports · 2025Article
- Relationship between corneal hysteresis and the site of damage to peripapillary retinal nerve fibre layer thickness in open-angle glaucoma.Scientific reports · 2024Article
- Pivotal Role of Corneal Fibroblasts in Progression to Corneal Ulcer in Bacterial Keratitis.International journal of molecular sciences · 2021Review
- Corneal stromal wound healing: Major regulators and therapeutic targets.The ocular surface · 2021Review
- The association between oxidative stress and corneal hysteresis in patients with glaucoma.Scientific reports · 2020Article
- Collagen at the maternal-fetal interface in human pregnancy.International journal of biological sciences · 2020Review
- The effect of estrogen and progesterone on porcine corneal biomechanical properties.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Is sex a biological variable in corneal wound healing?Experimental eye research · 2019Article
- Interstitial fibrosis in papillary thyroid microcarcinoma and its association with biological behavior.Oncology letters · 2018Article
- The impact of combined oral contraceptives on ocular tissues: a review of ocular effects.International journal of ophthalmology · 2017Review
- Role of corneal collagen fibrils in corneal disorders and related pathological conditions.International journal of ophthalmology · 2017Review
- Inhibitory Effect of Bone Morphogenetic Protein 4 in Retinal Pigment Epithelial-Mesenchymal Transition.Scientific reports · 2016Article
- Differential responses to steroid hormones in fibroblasts from the vocal fold, trachea, and esophagus.Endocrinology · 2015Article
- Molecular mechanism of the inhibition effect of Lipoxin A4 on corneal dissolving pathology process.International journal of ophthalmology · 2013Article
- Molecular mechanism of the inhibition effect of Celecoxib on corneal collagen degradation in three dimensions.International journal of ophthalmology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeCorneal fibroblasts contribute to collagen remodeling in the corneal stroma in part by mediating collagen degradation. Given that corneal structure is influenced by sex hormone status, we examined the effects of sex hormones on collagen degradation by corneal fibroblasts.
methodsRabbit corneal fibroblasts were cultured in three-dimensional collagen gels with or without sex hormones including 17β-estradiol, progesterone, testosterone, and dehydroepiandrosterone (DHEA). Collagen degradation was determined by measurement of hydroxyproline after acid hydrolysis. The expression and activity of matrix metalloproteinases (MMPs) were evaluated by immunoblot analysis and gelatin zymography. The phosphorylation of mitogen-activated protein kinases (MAPKs) and the nuclear factor-kappa B (NF-κB) inhibitor NF kappa B Inhibitor-alpha (IκB-α) in corneal fibroblasts was examined by immunoblot analysis. Cell proliferation and viability were evaluated by measurement of bromodeoxyuridine incorporation and the release of lactate dehydrogenase, respectively.
results17β-Estradiol and progesterone each inhibited interleukin (IL)-1β-induced collagen degradation by corneal fibroblasts in a concentration-dependent manner, whereas testosterone and DHEA had no such effect. MMP expression and activation in corneal fibroblasts exposed to IL-1β were also inhibited by 17β-estradiol and progesterone. These female sex hormones did not affect cell proliferation or viability. Both 17β-estradiol and progesterone inhibited the IL-1β-induced phosphorylation of p38 MAPK without affecting that of the MAPKs extracellular Signal-regulated Kinase (ERK) or c-jun N-terminal kinase (JNK). 17β-Estradiol also inhibited the IL-1β-induced phosphorylation of IκB-α.
conclusions17β-Estradiol and progesterone inhibited MMP expression and activity in IL-1β-stimulated corneal fibroblasts and thereby suppressed collagen degradation by these cells.
Indexed as
Identifiers
22219637PMC3249437W1543443896What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.