ArticleMolecular biology of the cell2012
Single-molecule imaging of translational output from individual RNA granules in neurons.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 63 citations in OpenAlex.
- Review
- Calibrated ribosome profiling assesses the dynamics of ribosomal flux on transcripts.Nature communications · 2024Article
- Development of single-molecule ubiquitination mediated fluorescence complementation to visualize protein ubiquitination dynamics in dendrites.Cell reports · 2022Article
- Molecular physiology of Arc/Arg3.1: The oligomeric state hypothesis of synaptic plasticity.Acta physiologica (Oxford, England) · 2022Review
- Are there roles for heterogeneous ribosomes during sleep in the rodent brain?Frontiers in molecular biosciences · 2022Article
- mRNA Targeting, Transport and Local Translation in Eukaryotic Cells: From the Classical View to a Diversity of New Concepts.Molecular biology · 2021Article
- A native function for RAN translation and CGG repeats in regulating fragile X protein synthesis.Nature neuroscience · 2020Article
- Article
- Local translation in neurons: visualization and function.Nature structural & molecular biology · 2019Review
- Fluorescence Imaging Methods to Investigate Translation in Single Cells.Cold Spring Harbor perspectives in biology · 2019Review
- Quantifying Single mRNA Translation Kinetics in Living Cells.Cold Spring Harbor perspectives in biology · 2018Review
- Aberrant RNA translation in fragile X syndrome: From FMRP mechanisms to emerging therapeutic strategies.Brain research · 2018Review
- Roles for Arc in metabotropic glutamate receptor-dependent LTD and synapse elimination: Implications in health and disease.Seminars in cell & developmental biology · 2018Review
- Article
- Multivalent Molecules as Modulators of RNA Granule Size and Composition.Biophysical journal · 2017Article
- Single Molecule Translation Imaging Visualizes the Dynamics of Local β-Actin Synthesis in Retinal Axons.Scientific reports · 2017Article
- Screening the Molecular Framework Underlying Local Dendritic mRNA Translation.Frontiers in molecular neuroscience · 2017Review
- Real-Time Imaging Reveals Properties of Glutamate-Induced Arc/Arg 3.1 Translation in Neuronal Dendrites.Neuron · 2016Article
- RNA assemblages orchestrate complex cellular processes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Dendritic RNAs are localized and translated in RNA granules. Here we use single-molecule imaging to count the number of RNA molecules in each granule and to record translation output from each granule using Venus fluorescent protein as a reporter. For RNAs encoding activity-regulated cytoskeletal-associated protein (ARC) or fragile X mental retardation protein (FMRP), translation events are spatially clustered near individual granules, and translational output from individual granules is either sporadic or bursty. The probability of bursty translation is greater for Venus-FMRP RNA than for Venus-ARC RNA and is increased in Fmr1-knockout neurons compared to wild-type neurons. Dihydroxyphenylglycine (DHPG) increases the rate of sporadic translation and decreases bursty translation for Venus-FMRP and Venus-ARC RNAs. Single-molecule imaging of translation in individual granules provides new insight into molecular, spatial, and temporal regulation of translation in granules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.