ArticleThe Journal of physiology2012
Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- Astrocyte-induced firing in primary afferent axons.iScience · 2025Article
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Application of optogenetic glial cells to neuron-glial communication.Frontiers in cellular neuroscience · 2023Review
- The Effects of Optogenetic Activation of Astrocytes on Spike-and-Wave Discharges in Genetic Absence Epileptic Rats.Annals of neurosciences · 2022Article
- Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of CaCells · 2021Review
- Novel Approaches Used to Examine and Control Neurogenesis in Parkinson's Disease.International journal of molecular sciences · 2021Review
- Ion Channels and Electrophysiological Properties of Astrocytes: Implications for Emergent Stimulation Technologies.Frontiers in cellular neuroscience · 2021Review
- Improved tools to study astrocytes.Nature reviews. Neuroscience · 2020Review
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- Optical control of neuronal ion channels and receptors.Nature reviews. Neuroscience · 2019Review
- Holographic two-photon activation for synthetic optogenetics.Nature protocols · 2019Article
- Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation.Nature communications · 2019Article
- Exploring the Role of Astroglial Glutamate Release and Association With Synapses in Neuronal Function and Behavior.Biological psychiatry · 2018Review
- Gliotransmission and adenosinergic modulation: insights from mammalian spinal motor networks.Journal of neurophysiology · 2017Review
- An autocrine purinergic signaling controls astrocyte-induced neuronal excitation.Scientific reports · 2017Article
- Synapses in the spotlight with synthetic optogenetics.EMBO reports · 2017Review
- Astroglial Glutamate Signaling and Uptake in the Hippocampus.Frontiers in molecular neuroscience · 2017Review
- Optogenetic and Chemogenetic Approaches for Studying Astrocytes and Gliotransmitters.Experimental neurobiology · 2016Review
- Calcium Uncaging with Visible Light.Journal of the American Chemical Society · 2016Article
- A family of photoswitchable NMDA receptors.eLife · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Increases in astrocyte Ca(2+) have been suggested to evoke gliotransmitter release, however, the mechanism of release, the identity of such transmitter(s), and even whether and when such release occurs, are controversial, largely due to the lack of a method for selective and reproducible stimulation of electrically silent astrocytes. Here we show that photoactivation of the light-gated Ca(2+)-permeable ionotropic GluR6 glutamate receptor (LiGluR), and to a lesser extent the new Ca(2+)-translocating channelrhodopsin CatCh, evokes more reliable Ca(2+) elevation than the mutant channelrhodopsin 2, ChR2(H134R) in cultured cortical astrocytes. We used evanescent-field excitation for near-membrane Ca(2+) imaging, and epifluorescence to activate and inactivate LiGluR. By alternating activation and inactivation light pulses, the LiGluR-evoked Ca(2+) rises could be graded in amplitude and duration. The optical stimulation of LiGluR-expressing astrocytes evoked probabilistic glutamate-mediated signalling to adjacent LiGluR-non-expressing astrocytes. This astrocyte-to-astrocyte signalling was insensitive to the inactivation of vesicular release, hemichannels and glutamate-transporters, and sensitive to anion channel blockers. Our results show that LiGluR is a powerful tool to selectively and reproducibly activate astrocytes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.