ArticleThe Journal of biological chemistry2012
Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
- Article
- DNA Methylation Inhibition Reversibly Impairs the Long-Term Context Memory Maintenance inInternational journal of molecular sciences · 2023Article
- DNA methyltransferase activity in the basolateral amygdala is critical for reconsolidation of a heroin reward memory.Frontiers in molecular neuroscience · 2022Article
- Epigenetic regulation of RNA polymerase III transcription in early breast tumorigenesis.Oncogene · 2017Article
- Interplay between epigenetics and metabolism in oncogenesis: mechanisms and therapeutic approaches.Oncogene · 2017Review
- Gender Specific Differences in RNA Polymerase III Transcription.Journal of carcinogenesis & mutagenesis · 2016Article
- Reconsolidation of a cocaine associated memory requires DNA methyltransferase activity in the basolateral amygdala.Scientific reports · 2015Article
- The B-WICH chromatin-remodelling complex regulates RNA polymerase III transcription by promoting Max-dependent c-Myc binding.Nucleic acids research · 2015Article
- SINE transcription by RNA polymerase III is suppressed by histone methylation but not by DNA methylation.Nature communications · 2015Article
- Inhibition of DNA methyltransferase as a novel therapeutic strategy to overcome acquired resistance to dual PI3K/mTOR inhibitors.Oncotarget · 2015Article
- nc886, a non-coding RNA of anti-proliferative role, is suppressed by CpG DNA methylation in human gastric cancer.Oncotarget · 2014Article
- Emerging landscape of non-coding RNAs in oral health and disease.Oral diseases · 2014Review
- Contributions of in vitro transcription to the understanding of human RNA polymerase III transcription.Transcription · 2014Review
- The functional role of long non-coding RNAs and epigenetics.Biological procedures online · 2014Review
- Going Green: The Role of the Green Tea Component EGCG in Chemoprevention.Journal of carcinogenesis & mutagenesis · 2013Article
- Genome-wide analysis of DNA methylation and expression of microRNAs in breast cancer cells.International journal of molecular sciences · 2012Article
- RNA polymerase III repression by the retinoblastoma tumor suppressor protein.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The human small nuclear RNA (snRNA) and small cytoplasmic RNA (scRNA) gene families encode diverse non-coding RNAs that influence cellular growth and division. Many snRNA and scRNA genes are related via their compact and yet powerful promoters that support RNA polymerase III transcription. We have utilized the human U6 snRNA gene family to examine the mechanism for regulated transcription of these potent transcription units. Analysis of nine U6 family members showed enriched CpG density within the promoters of actively transcribed loci relative to inert genes, implying a relationship between gene potency and DNA methylation. Indeed, both pharmacological inhibition of DNA methyltransferase (DNMT) activity and the forced diminution of DNMT-1, DNMT-3a, and DNMT-3b by siRNA targeting resulted in increased U6 levels in asynchronously growing MCF7 adenocarcinoma cells. In vitro transcription assays further showed that template methylation impedes U6 transcription by RNA polymerase III. Both DNMT-1 and DNMT-3a were detected at the U6-1 locus by chromatin immunoprecipitation directly linking these factors to RNA polymerase III regulation. Despite this association, the endogenous U6-1 locus was not substantially methylated in actively growing cells. However, both DNMT occupancy and low frequency methylation were correlated with increased Retinoblastoma tumor suppressor (RB) expression, suggesting that the RB status can influence specific epigenetic marks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.