ArticleMolecular and cellular biochemistry2012
Expression and role in glycolysis of human ADP-dependent glucokinase.
Article in Molecular and cellular biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Revisiting the Glucose Kinase Superfamily: From Origins to the Evolution of Metabolism.Genome biology and evolution · 2025Article
- Discovering plasma proteins associated with breast cancer incidence in postmenopausal women in the Atherosclerosis Risk in Communities study.Journal of the National Cancer Institute · 2025Article
- Machine learning-based analysis identifies glucose metabolism-related genes ADPGK as potential diagnostic biomarkers for clear cell renal cell carcinoma.Frontiers in oncology · 2025Article
- Emerging roles of ADP-dependent glucokinase in prostate cancer.Military Medical Research · 2024Article
- Current status and progress of research on the ADP-dependent glucokinase gene.Frontiers in oncology · 2024Review
- ADP-dependent glucokinase controls metabolic fitness in prostate cancer progression.Military Medical Research · 2023Article
- Review
- Hypothesis: A Novel Neuroprotective Role for Glucose-6-phosphatase (G6PC3) in Brain-To Maintain Energy-Dependent Functions Including Cognitive Processes.Neurochemical research · 2020Review
- ADP-dependent glucokinase as a novel onco-target for haematological malignancies.Scientific reports · 2020Article
- ADP-dependent glucokinase regulates energy metabolism via ER-localized glucose sensing.Scientific reports · 2019Article
- Adventitial Cell Atlas of wt (Wild Type) and ApoE (Apolipoprotein E)-Deficient Mice Defined by Single-Cell RNA Sequencing.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- De novo transcriptome sequencing and analysis revealed the molecular basis of rapid fat accumulation by black soldier fly (Biotechnology for biofuels · 2019Article
- Structural basis for ADP-dependent glucokinase inhibition by 8-bromo-substituted adenosine nucleotide.The Journal of biological chemistry · 2018Article
- Crystal structure of ADP-dependent glucokinase from Methanocaldococcus jannaschii in complex with 5-iodotubercidin reveals phosphoryl transfer mechanism.Protein science : a publication of the Protein Society · 2018Article
- Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases.The Journal of biological chemistry · 2017Article
- Chemoproteomic Profiling of Bile Acid Interacting Proteins.ACS central science · 2017Article
- Benserazide, a dopadecarboxylase inhibitor, suppresses tumor growth by targeting hexokinase 2.Journal of experimental & clinical cancer research : CR · 2017Article
- Genetic Dissection of a QTL Affecting Bone Geometry.G3 (Bethesda, Md.) · 2017Article
- The Structural and Functional Characterization of Mammalian ADP-dependent Glucokinase.The Journal of biological chemistry · 2016Article
- Immunometabolism in Tuberculosis.Frontiers in immunology · 2016Review
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel murine enzyme, ADP-dependent glucokinase (ADPGK), has been shown to catalyse glucose phosphorylation using ADP as phosphoryl donor. The ancestral ADPGK gene appears to have been laterally transferred from Archaea early in metazoan evolution, but its biological role has not been established. Here, we undertake an initial investigation of the functional properties of human ADPGK in human tumour cell lines and specifically test the hypothesis that ADPGK might prime glycolysis using ADP under stress conditions such as hypoxia. Recombinant human ADPGK was confirmed to catalyse ADP-dependent glucose phosphorylation in vitro, with an apparent K (M) for glucose of 0.29 mM. Expression databases and western blotting of surgical samples demonstrated high expression in many human tissues, including tumours. Unlike hexokinase-2 (HK2), RNAi studies with exon arrays showed that ADPGK is not a transcriptional target of hypoxia inducible factor-1. Consistent with this, ADPGK protein was not upregulated by hypoxia or anoxia. Surprisingly, stable fivefold overexpression of ADPGK in H460 or HCT116 cells had no apparent effect on proliferation or glycolysis, and did not rescue clonogenicity or glycolysis when HK2 was suppressed by siRNA. Furthermore, suppression of ADPGK by siRNA did not cause detectable inhibition of glycolysis or cell killing by anoxia, although it did induce a statistically significant decrease in plating efficiency of H460 cells under aerobic conditions. Thus, human ADPGK catalyses ADP-dependent phosphorylation of glucose in vitro, but despite its high expression in human tumour cell lines it appears not to make a quantifiable contribution to glycolysis under the conditions evaluated.
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