ArticleMethods in molecular biology (Clifton, N.J.)2012
Gene-centered yeast one-hybrid assays.
Article in Methods in molecular biology (Clifton, N.J.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- A rapid test for protein-DNA interactions.Nucleic acids research · 2026Article
- Construction of Yeast One-Hybrid Library ofJournal of fungi (Basel, Switzerland) · 2023Article
- The transcription factor TaMYB31 regulates the benzoxazinoid biosynthetic pathway in wheat.Journal of experimental botany · 2022Article
- Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system.Nucleic acids research · 2018Article
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- A gene-centered C. elegans protein-DNA interaction network provides a framework for functional predictions.Molecular systems biology · 2016Article
- Potential Direct Regulators of the Drosophila yellow Gene Identified by Yeast One-Hybrid and RNAi Screens.G3 (Bethesda, Md.) · 2016Article
- Article
- Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6.BMC developmental biology · 2014Article
- Transcription factor binding to Caenorhabditis elegans first introns reveals lack of redundancy with gene promoters.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transcription is regulated by sequence-specific transcription factors (TFs) that bind to short genomic DNA elements that can be located in promoters, enhancers and other cis-regulatory modules. Determining which TFs bind where requires techniques that enable the ab initio identification of TF-DNA interactions. These techniques can either be "TF-centered" (protein-to-DNA), where regions of DNA bound by a TF of interest are identified, or "gene-centered" (DNA-to-protein), where TFs that bind a DNA sequence of interest are identified. Here, we describe gene-centered yeast one-hybrid (Y1H) assays. Briefly, in Y1H assays, a DNA fragment is cloned upstream of two different reporters, and these reporter constructs are integrated into the genome of a yeast strain. Next, plasmids expressing TFs as hybrid proteins (hence the name of the assay) fused with the strong transcriptional activation domain (AD) of the yeast TF Gal4 are introduced into the yeast strain. When a TF interacts with the DNA fragment of interest, the AD moiety activates reporter expression in yeast regardless of whether the TF is an activator or repressor in vivo. Sequencing the plasmid in the colonies that exhibit reporter activation reveals the identity of the TFs that can bind the DNA fragment. We have shown Y1H to be a robust method for detecting interactions between a variety of DNA elements and multiple families of TFs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.