ArticlePloS one2011
Effect of the Salmonella pathogenicity island 2 type III secretion system on Salmonella survival in activated chicken macrophage-like HD11 cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
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- CCL4 participates in the reprogramming of glucose metabolism induced by ALV-J infection in chicken macrophages.Frontiers in microbiology · 2023Article
- High Affinity Iron Acquisition Systems Facilitate but Are Not Essential for Colonization of Chickens byFrontiers in microbiology · 2022Article
- Transcriptome Analyses of Chicken Primary Macrophages Infected With AttenuatedFrontiers in microbiology · 2022Article
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- The Differential Phosphorylation-Dependent Signaling and Glucose Immunometabolic Responses Induced during Infection byMicroorganisms · 2020Article
- Temperature-Dependent Gentamicin Resistance of Francisella tularensis is Mediated by Uptake Modulation.Frontiers in microbiology · 2016Article
- Immune reaction and survivability of salmonella typhimurium and salmonella infantis after infection of primary avian macrophages.PloS one · 2015Article
- Salmonella enterica serovar enteritidis antimicrobial peptide resistance genes aid in defense against chicken innate immunity, fecal shedding, and egg deposition.Infection and immunity · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to better identify the role of the Salmonella pathogenicity island 2 (SPI-2) type III secretion system (T3SS) in chickens, we used the well-known gentamicin protection assay with activated HD11 cells. HD11 cells are a macrophage-like chicken cell line that can be stimulated with phorbol 12-myristate 13-acetate (PMA) to exhibit more macrophage-like morphology and greater production of reactive oxygen species (ROS). Activated HD11 cells were infected with a wild-type Salmonella enterica subspecies enterica serovar Typhimurium (S. Typhimurium) strain, a SPI-2 mutant S. Typhimurium strain, a wild-type Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) strain, a SPI-2 mutant S. Enteritidis strain, or a non-pathogenic Escherichia coli (E. coli) strain. SPI-2 mutant strains were found to survive as well as their parent strain at all time points post-uptake (PU) by the HD11 cells, up to 24 h PU, while the E. coli strain was no longer recoverable by 3 h PU. We can conclude from these observations that the SPI-2 T3SS of S. Typhimurium and S. Enteritidis is not important for survival of Salmonella in the activated macrophage-like HD11 cell line, and that Salmonella must employ other mechanisms for survival in this environment, as E. coli is effectively eliminated.
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