Evidence map›Paper›PMID 22216251›Full record

ArticlePloS one2011

Genome-wide identification of transcriptional start sites in the plant pathogen Pseudomonas syringae pv. tomato str. DC3000.

Melanie J Filiatrault, Paul V Stodghill, Christopher R Myers, Philip A Bronstein, Bronwyn G Butcher, Hanh Lam, George Grills, Peter Schweitzer, Wei Wang, David J Schneider and 1 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
6.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 35 citations in OpenAlex.

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  4. CaJournal of bacteriology · 2018
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  17. Catalyzing plant science research with RNA-seq.Frontiers in plant science · 2013
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Melanie J Filiatraultlant-Microbe Interactions Research Unit, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, New York, USA. Melanie.filiatrault@ars.usda.gov
Paul V Stodghill
Christopher R Myers
Philip A Bronstein
Bronwyn G Butcher
Hanh Lam
George Grills
Peter Schweitzer
Wei Wang
David J Schneider
Samuel W Cartinhour
Cornell University · USUnited States Department of Agriculture · USRobert W. Holley Center for Agriculture & Health · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RNA-Seq has provided valuable insights into global gene expression in a wide variety of organisms. Using a modified RNA-Seq approach and Illumina's high-throughput sequencing technology, we globally identified 5'-ends of transcripts for the plant pathogen Pseudomonas syringae pv. tomato str. DC3000. A substantial fraction of 5'-ends obtained by this method were consistent with results obtained using global RNA-Seq and 5'RACE. As expected, many 5'-ends were positioned a short distance upstream of annotated genes. We also captured 5'-ends within intergenic regions, providing evidence for the expression of un-annotated genes and non-coding RNAs, and detected numerous examples of antisense transcription, suggesting additional levels of complexity in gene regulation in DC3000. Importantly, targeted searches for sequence patterns in the vicinity of 5'-ends revealed over 1200 putative promoters and other regulatory motifs, establishing a broad foundation for future investigations of regulation at the genomic and single gene levels.

Indexed as

Genome, PlantTranscription, GeneticBase SequenceDNA PrimersPseudomonas syringaeReproducibility of ResultsRNA, PlantSolanum lycopersicumDNA PrimersRNA, Plant

Identifiers

PMID22216251
PMCPMC3247240
OpenAlexW2137464298

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.