ArticlePloS one2011
Differential gene expression from microarray analysis distinguishes woven and lamellar bone formation in the rat ulna following mechanical loading.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
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- Modulation of TRPV4 protects against degeneration induced by sustained loading and promotes matrix synthesis in the intervertebral disc.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.Bone · 2022Article
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- Transcriptional profiling of intramembranous and endochondral ossification after fracture in mice.Bone · 2019Article
- The Interaction of BMP2-Induced Defect Healing in Rat and Fixator Stiffness Modulates Matrix Alignment and Contraction.JBMR plus · 2018Article
- Mechanical instability and titanium particles induce similar transcriptomic changes in a rat model for periprosthetic osteolysis and aseptic loosening.Bone reports · 2017Article
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- Effects of Deletion of ERα in Osteoblast-Lineage Cells on Bone Mass and Adaptation to Mechanical Loading Differ in Female and Male Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
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- Antagonizing the αv β3 integrin inhibits angiogenesis and impairs woven but not lamellar bone formation induced by mechanical loading.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways.Journal of biomechanics · 2014Article
- Nitric oxide-mediated vasodilation increases blood flow during the early stages of stress fracture healing.Journal of applied physiology (Bethesda, Md. : 1985) · 2014Article
- Osteocyte expression of caspase-3, COX-2, IL-6 and sclerostin are spatially and temporally associated following stress fracture initiation.BoneKEy reports · 2014Article
- Skeletal Blood Flow in Bone Repair and Maintenance.Bone research · 2013Review
- Gene expression responses to mechanical stimulation of mesenchymal stem cells seeded on calcium phosphate cement.Tissue engineering. Part A · 2013Article
- Biophysical regulation of stem cell differentiation.Current osteoporosis reports · 2013Review
- Adaptive and Injury Response of Bone to Mechanical Loading.BoneKEy osteovision · 2012Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Formation of woven and lamellar bone in the adult skeleton can be induced through mechanical loading. Although much is known about the morphological appearance and structural properties of the newly formed bone, the molecular responses to loading are still not well understood. The objective of our study was to use a microarray to distinguish the molecular responses between woven and lamellar bone formation induced through mechanical loading. Rat forelimb loading was completed in a single bout to induce the formation of woven bone (WBF loading) or lamellar bone (LBF loading). A set of normal (non-loaded) rats were used as controls. Microarrays were performed at three timepoints after loading: 1 hr, 1 day and 3 days. Confirmation of microarray results was done for a select group of genes using quantitative real-time PCR (qRT-PCR). The micorarray identified numerous genes and pathways that were differentially regulated for woven, but not lamellar bone formation. Few changes in gene expression were evident comparing lamellar bone formation to normal controls. A total of 395 genes were differentially expressed between formation of woven and lamellar bone 1 hr after loading, while 5883 and 5974 genes were differentially expressed on days 1 and 3, respectively. Results suggest that not only are the levels of expression different for each type of bone formation, but that distinct pathways are activated only for woven bone formation. A strong early inflammatory response preceded an increase in angiogenic and osteogenic gene expression for woven bone formation. Furthermore, at later timepoints there was evidence of bone resorption after WBF loading. In summary, the vast coverage of the microarray offers a comprehensive characterization of the early differences in expression between woven and lamellar bone formation.
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