ArticlePloS one2011
The effects of mechanical stress on the growth, differentiation, and paracrine factor production of cardiac stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 56 citations in OpenAlex.
- "Advancing cardiac patch viability and functionality: innovations in scaffold design and cellular optimization".Journal of materials science. Materials in medicine · 2025Review
- Can repetitive mechanical motion cause structural damage to axons?Frontiers in molecular neuroscience · 2024Review
- OctoShaker: A versatile robotic biomechanical agitator for cellular and organoid research.The Review of scientific instruments · 2023Article
- Effects of Mechanical Stress on Endothelial Cells In Situ and In Vitro.International journal of molecular sciences · 2023Review
- Directionality of developing skeletal muscles is set by mechanical forces.Nature communications · 2023Article
- Potential Role of TRPV4 in Stretch-Induced Ghrelin Secretion and Obesity.International journal of endocrinology · 2022Article
- Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Recent Advances in Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Promoted by Mechanical Stretch.Medical science monitor : international medical journal of experimental and clinical research · 2021Review
- [Application of hemodynamic optimization in the design of artificial heart].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2020Article
- Review
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- The effects of mechanical stretch on the biological characteristics of human adipose-derived stem cells.Journal of cellular and molecular medicine · 2019Article
- Mechanical influences on cardiovascular differentiation and disease modeling.Experimental cell research · 2019Review
- Calcium-dependent potassium channels control proliferation of cardiac progenitor cells and bone marrow-derived mesenchymal stem cells.The Journal of physiology · 2018Article
- Evaluation of Hydrogels Presenting Extracellular Matrix-Derived Adhesion Peptides and Encapsulating Cardiac Progenitor Cells for Cardiac Repair.ACS biomaterials science & engineering · 2018Article
- The productivity limit of manufacturing blood cell therapy in scalable stirred bioreactors.Journal of tissue engineering and regenerative medicine · 2018Article
- Low-level stretching accelerates cell migration into a gap.International wound journal · 2017Article
- Origin of cardiomyocytes in the adult heart.Circulation research · 2015Review
- Advanced Strategies for End-Stage Heart Failure: Combining Regenerative Approaches with LVAD, a New Horizon?Frontiers in surgery · 2015Review
- Making it stick: chasing the optimal stem cells for cardiac regeneration.Expert review of cardiovascular therapy · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cell therapies have been clinically employed to repair the injured heart, and cardiac stem cells are thought to be one of the most potent stem cell candidates. The beating heart is characterized by dynamic mechanical stresses, which may have a significant impact on stem cell therapy. The purpose of this study is to investigate how mechanical stress affects the growth and differentiation of cardiac stem cells and their release of paracrine factors. In this study, human cardiac stem cells were seeded in a silicon chamber and mechanical stress was then induced by cyclic stretch stimulation (60 cycles/min with 120% elongation). Cells grown in non-stretched silicon chambers were used as controls. Our result revealed that mechanical stretching significantly reduced the total number of surviving cells, decreased Ki-67-positive cells, and increased TUNEL-positive cells in the stretched group 24 hrs after stretching, as compared to the control group. Interestingly, mechanical stretching significantly increased the release of the inflammatory cytokines IL-6 and IL-1β as well as the angiogenic growth factors VEGF and bFGF from the cells in 12 hrs. Furthermore, mechanical stretching significantly reduced the percentage of c-kit-positive stem cells, but increased the expressions of cardiac troponin-I and smooth muscle actin in cells 3 days after stretching. Using a traditional stretching model, we demonstrated that mechanical stress suppressed the growth and proliferation of cardiac stem cells, enhanced their release of inflammatory cytokines and angiogenic factors, and improved their myogenic differentiation. The development of this in vitro approach may help elucidate the complex mechanisms of stem cell therapy for heart failure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.