ArticlePloS one2011
A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Optimizing methods for virome analysis based on studies of a synthetic viral community.mSystems · 2026Article
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- Optimizing methods for virome analysis based on studies of a synthetic viral community.bioRxiv : the preprint server for biology · 2025Article
- Screening for Viral Nucleic Acids in the Cerebrospinal Fluid of Dogs With Central Nervous System Inflammation.Frontiers in veterinary science · 2022Article
- A Versatile Processing Workflow to Enable Pathogen Detection in Clinical Samples from Organs Using VIDISCA.Diagnostics (Basel, Switzerland) · 2021Article
- Implementation of next-generation sequencing for virus identification in veterinary diagnostic laboratories.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- The Diagnostic Value of Metagenomic Next-Generation Sequencing in Lower Respiratory Tract Infection.Frontiers in cellular and infection microbiology · 2021Review
- Broad Virus Detection and Variant Discovery in Fecal Samples of Hematopoietic Transplant Recipients Using Targeted Sequence Capture Metagenomics.Frontiers in microbiology · 2020Article
- Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology.Scientific reports · 2019Article
- The Promises and Pitfalls of Machine Learning for Detecting Viruses in Aquatic Metagenomes.Frontiers in microbiology · 2019Article
- Laboratory Methods in Molecular Epidemiology: Viral Infections.Microbiology spectrum · 2018Review
- Evaluation of bias induced by viral enrichment and random amplification protocols in metagenomic surveys of saliva DNA viruses.Microbiome · 2018Article
- Complete Genome Sequence of a Swiss Hepatitis E Virus Isolate from the Liver of a Fattening Pig.Genome announcements · 2018Article
- Viral metagenomics of six bat species in close contact with humans in southern China.Archives of virology · 2018Article
- Viral Enrichment Methods Affect the Detection but Not Sequence Variation of West Nile Virus in Equine Brain Tissue.Frontiers in veterinary science · 2018Article
- Complete Genome Sequences of Two Swiss Hepatitis E Virus Isolates from Human Stool and Raw Pork Sausage.Genome announcements · 2017Article
- Evolution of selective-sequencing approaches for virus discovery and virome analysis.Virus research · 2017Review
- Efficient and unbiased metagenomic recovery of RNA virus genomes from human plasma samples.Scientific reports · 2017Article
- Evaluation of Methods for the Concentration and Extraction of Viruses from Sewage in the Context of Metagenomic Sequencing.PloS one · 2017Article
- Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays.Science advances · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.