ArticlePloS one2011
Carbohydrate metabolism is essential for the colonization of Streptococcus thermophilus in the digestive tract of gnotobiotic rats.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Improving lactose digestion and symptoms of lactose intolerance with a novel galacto-oligosaccharide (RP-G28): a randomized, double-blind clinical trial.Nutrition journal · 2013Trial
- Interplay between host genetics and gut microbiome composition in the Japanese population.Frontiers in microbiomes · 2025Article
- Use of Rgg quorum-sensing machinery to create an innovative recombinant protein expression system inMicrobiology (Reading, England) · 2024Article
- Influence of Lactose Supplementation on Regulation ofNutrients · 2023Article
- Genetic and technological diversity ofFrontiers in microbiology · 2023Article
- Identification ofMicroorganisms · 2021Article
- Comparative Genomic and Functional Evaluations ofFoods (Basel, Switzerland) · 2020Article
- Enterococcus faecalis Maltodextrin Gene Regulation by Combined Action of Maltose Gene Regulator MalR and Pleiotropic Regulator CcpA.Applied and environmental microbiology · 2020Article
- Characterization of Mucus-Related Properties ofFrontiers in physiology · 2018Article
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- New Insights into Various Production Characteristics of Streptococcus thermophilus Strains.International journal of molecular sciences · 2016Review
- Anoxic Conditions Promote Species-Specific Mutualism between Gut Microbes In Silico.Applied and environmental microbiology · 2015Article
- Early colonizing Escherichia coli elicits remodeling of rat colonic epithelium shifting toward a new homeostatic state.The ISME journal · 2015Article
- Article
- Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.PloS one · 2013Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus thermophilus is the archetype of lactose-adapted bacterium and so far, its sugar metabolism has been mainly investigated in vitro. The objective of this work was to study the impact of lactose and lactose permease on S. thermophilus physiology in the gastrointestinal tract (GIT) of gnotobiotic rats. We used rats mono-associated with LMD-9 strain and receiving 4.5% lactose. This model allowed the analysis of colonization curves of LMD-9, its metabolic profile, its production of lactate and its interaction with the colon epithelium. Lactose induced a rapid and high level of S. thermophilus in the GIT, where its activity led to 49 mM of intra-luminal L-lactate that was related to the induction of mono-carboxylic transporter mRNAs (SLC16A1 and SLC5A8) and p27(Kip1) cell cycle arrest protein in epithelial cells. In the presence of a continuous lactose supply, S. thermophilus recruited proteins involved in glycolysis and induced the metabolism of alternative sugars as sucrose, galactose, and glycogen. Moreover, inactivation of the lactose transporter, LacS, delayed S. thermophilus colonization. Our results show i/that lactose constitutes a limiting factor for colonization of S. thermophilus, ii/that activation of enzymes involved in carbohydrate metabolism constitutes the metabolic signature of S. thermophilus in the GIT, iii/that the production of lactate settles the dialogue with colon epithelium. We propose a metabolic model of management of carbohydrate resources by S. thermophilus in the GIT. Our results are in accord with the rationale that nutritional allegation via consumption of yogurt alleviates the symptoms of lactose intolerance.
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