ArticleInfection and immunity2012
Analysis of global transcriptional profiles of enterotoxigenic Escherichia coli isolate E24377A.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- Intestinal Epithelial Cells Modulate the Production of Enterotoxins by Porcine EnterotoxigenicInternational journal of molecular sciences · 2022Article
- Comparative Pathogenomics ofInfection and immunity · 2021Article
- Long-read-sequenced reference genomes of the seven major lineages of enterotoxigenic Escherichia coli (ETEC) circulating in modern time.Scientific reports · 2021Article
- Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Standardized phylogenetic and molecular evolutionary analysis applied to species across the microbial tree of life.Scientific reports · 2020Article
- Comparative genomic analysis and molecular examination of the diversity of enterotoxigenic Escherichia coli isolates from Chile.PLoS neglected tropical diseases · 2019Article
- The bile salt glycocholate induces global changes in gene and protein expression and activates virulence in enterotoxigenic Escherichia coli.Scientific reports · 2019Article
- Comparative genomic analysis of enterotoxigenicGut pathogens · 2019Article
- Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology.Scientific reports · 2017Article
- Diarrheagenic Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2016Review
- Survival of the Fittest: How Bacterial Pathogens Utilize Bile To Enhance Infection.Clinical microbiology reviews · 2016Review
- The different ecological niches of enterotoxigenic Escherichia coli.Environmental microbiology · 2016Review
- Comparative Genomics Provides Insight into the Diversity of the Attaching and Effacing Escherichia coli Virulence Plasmids.Infection and immunity · 2015Article
- IscR Regulates Synthesis of Colonization Factor Antigen I Fimbriae in Response to Iron Starvation in Enterotoxigenic Escherichia coli.Journal of bacteriology · 2015Article
- Draft Genome Sequence of Enterotoxigenic Escherichia coli Strain E24377A, Obtained from a Tribal Drinking Water Source in India.Genome announcements · 2015Article
- RNA-Seq analysis of isolate- and growth phase-specific differences in the global transcriptomes of enteropathogenic Escherichia coli prototype isolates.Frontiers in microbiology · 2015Article
- The molecular basis for control of ETEC enterotoxin expression in response to environment and host.PLoS pathogens · 2015Article
- Identification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli.PloS one · 2015Article
- Draft Whole-Genome Sequences of 10 Serogroup O6 Enterotoxigenic Escherichia coli Strains.Genome announcements · 2014Article
- Identification of enterotoxigenic Escherichia coli (ETEC) clades with long-term global distribution.Nature genetics · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enterotoxigenic Escherichia coli (ETEC) is an important pathogenic variant (pathovar) of E. coli in developing countries from a human health perspective, causing significant morbidity and mortality. Previous studies have examined specific regulatory networks in ETEC, although little is known about the global effects of inter- and intrakingdom signaling on the expression of virulence and colonization factors in ETEC. In this study, an E. coli/Shigella pan-genome microarray, combined with quantitative reverse transcriptase PCR (qRT-PCR) and RNA sequencing (RNA-seq), was used to quantify the expression of ETEC virulence and colonization factors. Biologically relevant chemical signals were combined with ETEC isolate E24377A during growth in either Luria broth (LB) or Dulbecco's modified Eagle medium (DMEM), and transcription was examined during different phases of the growth cycle; chemical signals examined included glucose, bile salts, and preconditioned media from E. coli/Shigella isolates. The results demonstrate that the presence of bile salts, which are found in the intestine and thought to be bactericidal, upregulates the expression of many ETEC virulence factors, including heat-stable (estA) and heat-labile (eltA) enterotoxin genes. In contrast, the ETEC colonization factors CS1 and CS3 were downregulated in the presence of bile, consistent with findings in studies of other enteric pathogens. RNA-seq analysis demonstrated that one of the most differentially expressed genes in the presence of bile is a unique plasmid-encoded AraC-like transcriptional regulator (peaR); other previously unknown genetic elements were found as well. These results provide transcriptional targets and putative mechanisms that should help improve understanding of the global regulatory networks and virulence expression in this important human pathogen.
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