ArticleThe Journal of biological chemistry2012
Adaptive evolution of class 5 fimbrial genes in enterotoxigenic Escherichia coli and its functional consequences.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Beyond FimH: Diversity and Relevance of Carbohydrate-Binding Fimbrial Proteins in Escherichia coli.Chembiochem : a European journal of chemical biology · 2025Review
- Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-ProducingMicroorganisms · 2022Article
- Development and Comparison of a Panel of Modified CS17 Fimbrial Tip Adhesin Proteins as Components for an Adhesin-Based Vaccine against EnterotoxigenicMicroorganisms · 2021Article
- Host-Pathogen Adhesion as the Basis of Innovative Diagnostics for Emerging Pathogens.Diagnostics (Basel, Switzerland) · 2021Review
- Interplay of Various Evolutionary Modes in Genome Diversification and Adaptive Evolution of the FamilyFrontiers in microbiology · 2021Article
- Article
- Mutational convergence acts as a major player in adaptive parallel evolution of Shigella spp.Scientific reports · 2019Article
- Recombination-independent rapid convergent evolution of the gastric pathogen Helicobacter pylori.BMC genomics · 2018Article
- A Role for Salivary Peptides in the Innate Defense Against Enterotoxigenic Escherichia coli.The Journal of infectious diseases · 2018Article
- Antibodies Damage the Resilience of Fimbriae, Causing Them To Be Stiff and Tangled.Journal of bacteriology · 2017Article
- Mutation-Driven Divergence and Convergence Indicate Adaptive Evolution of the Intracellular Human-Restricted Pathogen, Bartonella bacilliformis.PLoS neglected tropical diseases · 2016Article
- Binding of CFA/I Pili of Enterotoxigenic Escherichia coli to Asialo-GM1 Is Mediated by the Minor Pilin CfaE.Infection and immunity · 2016Article
- Simple method for purification of enterotoxigenic Escherichia coli fimbriae.Protein expression and purification · 2016Article
- Biomechanical and structural features of CS2 fimbriae of enterotoxigenic Escherichia coli.Biophysical journal · 2015Article
- Adhesion as a weapon in microbial competition.The ISME journal · 2015Article
- Allele variants of enterotoxigenic Escherichia coli heat-labile toxin are globally transmitted and associated with colonization factors.Journal of bacteriology · 2015Article
- Tracking recent adaptive evolution in microbial species using TimeZone.Nature protocols · 2013Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Class 5 fimbriae of enterotoxigenic Escherichia coli (ETEC) comprise eight serologically discrete colonization factors that mediate small intestinal adhesion. Their differentiation has been attributed to the pressure imposed by host adaptive immunity. We sequenced the major pilin and minor adhesin subunit genes of a geographically diverse population of ETEC elaborating CFA/I (n = 31), CS17 (n = 20), and CS2 (n = 18) and elucidated the functional effect of microevolutionary processes. Between the fimbrial types, the pairwise nucleotide diversity for the pilin or adhesin genes ranged from 35-43%. Within each fimbrial type, there were 17 non-synonymous and 1 synonymous point mutations among all pilin or adhesin gene copies, implying that each fimbrial type was acquired by ETEC strains very recently, consistent with a recent origin of this E. coli pathotype. The 17 non-synonymous allelic differences occurred in the CFA/I pilin gene cfaB (two changes) and adhesin gene cfaE (three changes), and CS17 adhesin gene csbD (12 changes). All but one amino acid change in the adhesins clustered around the predicted ligand-binding pocket. Functionally, these changes conferred an increase in cell adhesion in a flow chamber assay. In contrast, the two mutations in the non-adhesive CfaB subunit localized to the intersubunit interface and significantly reduced fimbrial adhesion in this assay. In conclusion, naturally occurring mutations in the ETEC adhesive and non-adhesive subunits altered function, were acquired under positive selection, and are predicted to impact bacteria-host interactions.
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Registered trials
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