ArticleThe Journal of biological chemistry2012
Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 151 papers, 1 of them a synthesis that pooled it.
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Who cites it
151 citing papers in PubMed, 1 synthesis or guideline pooled it, 303 citations in OpenAlex.
- Reciprocal responses in the interaction between Arabidopsis and the cell-content-feeding chelicerate herbivore spider mite.Plant physiology · 2014Pooled it
- The 11-hydroxy-jasmonic acid pathway expands jasmonate catabolic repertoire for stress-specific hormone attenuation in Arabidopsis.Nature communications · 2026Article
- Functional insights into a putative 2-OG oxygenase involved in jasmonic acid metabolism and xylem development in Populus trichocarpa.Plant & cell physiology · 2026Article
- Exogenous Methyl Jasmonate Enhances Heat Tolerance in Herbaceous Peony by Modulating Endogenous Hormone Homeostasis and the ROS Scavenging System.Antioxidants (Basel, Switzerland) · 2026Article
- Jasmonate Biosynthesis Across Bryophyte Lineages: Lessons fromPlants (Basel, Switzerland) · 2026Review
- (3R, 7S)-11-hydroxy-jasmonic acid is a major oxidative shunt product of jasmonic acid catabolism in Arabidopsis thaliana.Nature communications · 2026Article
- Silica nanoparticles enhance resistance of Vitis vinifera to downy mildew.BMC plant biology · 2026Article
- Molecular insights into volatile organic compound sensing and signaling in plants.The Plant journal : for cell and molecular biology · 2026Review
- Induction effect of exogenous arachidonic acid on resistance to white leaf spot in maize.BMC plant biology · 2026Article
- Genome-wide identification and expression profile ofFrontiers in plant science · 2026Article
- Transcription, Alternative Splicing, and Post-Translational Regulation of CaLOXs in the Dynamic Regulation of Jasmonate Levels in Wounded Pepper Leaves.Plants (Basel, Switzerland) · 2025Article
- Integrative Phytochemical and Transcriptomic Analysis Reveals Genes Regulating 14-Deoxyandrographolide in ThaiACS omega · 2025Article
- Robustness in jasmonate signaling: mechanisms of concerted regulation and implications for crop improvement.aBIOTECH · 2025Review
- Key genes and molecular mechanisms responsible for male sterility revealed by transcriptome analysis in cotton.Plant cell reports · 2025Article
- Ripening-induced defence signalling in Botrytis cinerea-infected tomato fruits involves activation of ERF.F4 by a MYC2-NOR/RIN protein complex.Plant biotechnology journal · 2025Article
- Article
- Salicylic acid and jasmonic acid in plant immunity.Horticulture research · 2025Article
- Wound-induced transcriptional dynamics in rice.Crop health · 2025Article
- Rice JASMONIC ACID OXIDASES control resting jasmonate metabolism to promote growth and repress basal immune responses.Plant physiology · 2025Article
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
91 more citing papers are in PubMed but not listed here.
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12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The jasmonate hormonal pathway regulates important defensive and developmental processes in plants. Jasmonoyl-isoleucine (JA-Ile) has been identified as a specific ligand binding the COI1-JAZ co-receptor to relieve repression of jasmonate responses. Two JA-Ile derivatives, 12OH-JA-Ile and 12COOH-JA-Ile, accumulate in wounded Arabidopsis leaves in a COI1- and JAR1-dependent manner and reflect catabolic turnover of the hormone. Here we report the biochemical and genetic characterization of two wound-inducible cytochromes P450, CYP94C1 and CYP94B3, that are involved in JA-Ile oxidation. Both enzymes expressed in yeast catalyze two successive oxidation steps of JA-Ile with distinct characteristics. CYP94B3 performed efficiently the initial hydroxylation of JA-Ile to 12OH-JA-Ile, with little conversion to 12COOH-JA-Ile, whereas CYP94C1 catalyzed preferentially carboxy-derivative formation. Metabolic analysis of loss- and gain-of-function plant lines were consistent with in vitro enzymatic properties. cyp94b3 mutants were largely impaired in 12OH-JA-Ile levels upon wounding and to a lesser extent in 12COOH-JA-Ile levels. In contrast, cyp94c1 plants showed wild-type 12OH-JA-Ile accumulation but lost about 60% 12COOH-JA-Ile. cyp94b3cyp94c1 double mutants hyperaccumulated JA-Ile with near abolition of 12COOH-JA-Ile. Distinct JA-Ile oxidation patterns in different plant genotypes were correlated with specific JA-responsive transcript profiles, indicating that JA-Ile oxidation status affects signaling. Interestingly, exaggerated JA-Ile levels were associated with JAZ repressor hyperinduction but did not enhance durably defense gene induction, revealing a novel negative feedback signaling loop. Finally, interfering with CYP94 gene expression affected root growth sensitivity to exogenous jasmonic acid. These results identify CYP94B3/C1-mediated oxidation as a major catabolic route for turning over the JA-Ile hormone.
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