ArticleThe Journal of biological chemistry2012
Luminal and cytosolic pH feedback on proton pump activity and ATP affinity of V-type ATPase from Arabidopsis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 40 citations in OpenAlex.
- Arabidopsis COP1 guides stomatal response in guard cells through pH regulation.Communications biology · 2024Article
- Homeostats: The hidden rulers of ion homeostasis in plants.Quantitative plant biology · 2024Review
- Transporter networks can serve plant cells as nutrient sensors and mimic transceptor-like behavior.iScience · 2022Article
- The Plant V-ATPase.Frontiers in plant science · 2022Review
- Nutrient cycling is an important mechanism for homeostasis in plant cells.Plant physiology · 2021Article
- Genomic insights into citrus domestication and its important agronomic traits.Plant communications · 2021Review
- Article
- The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes.Plant communications · 2020Article
- Dynamic measurement of cytosolic pH and [NOProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Plant Membrane Transport Research in the Post-genomic Era.Plant communications · 2020Review
- Voltage-dependent gating of SV channel TPC1 confers vacuole excitability.Nature communications · 2019Article
- Hyperacidification of Citrus fruits by a vacuolar proton-pumping P-ATPase complex.Nature communications · 2019Article
- High V-PPase activity is beneficial under high salt loads, but detrimental without salinity.The New phytologist · 2018Article
- Purification and functional characterization of the vacuolar malate transporter tDT fromThe Journal of biological chemistry · 2018Article
- Regulation of Vacuolar H+-ATPase (V-ATPase) Reassembly by Glycolysis Flow in 6-Phosphofructo-1-kinase (PFK-1)-deficient Yeast Cells.The Journal of biological chemistry · 2016Article
- Rapid structural changes and acidification of guard cell vacuoles during stomatal closure require phosphatidylinositol 3,5-bisphosphate.The Plant cell · 2013Article
- Fumarate and cytosolic pH as modulators of the synthesis or consumption of C(4) organic acids through NADP-malic enzyme in Arabidopsis thaliana.Plant molecular biology · 2013Article
- RNAi-directed downregulation of vacuolar H(+) -ATPase subunit a results in enhanced stomatal aperture and density in rice.PloS one · 2013Article
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9 authors at 5 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proton pumping of the vacuolar-type H(+)-ATPase into the lumen of the central plant organelle generates a proton gradient of often 1-2 pH units or more. Although structural aspects of the V-type ATPase have been studied in great detail, the question of whether and how the proton pump action is controlled by the proton concentration on both sides of the membrane is not understood. Applying the patch clamp technique to isolated vacuoles from Arabidopsis mesophyll cells in the whole-vacuole mode, we studied the response of the V-ATPase to protons, voltage, and ATP. Current-voltage relationships at different luminal pH values indicated decreasing coupling ratios with acidification. A detailed study of ATP-dependent H(+)-pump currents at a variety of different pH conditions showed a complex regulation of V-ATPase activity by both cytosolic and vacuolar pH. At cytosolic pH 7.5, vacuolar pH changes had relative little effects. Yet, at cytosolic pH 5.5, a 100-fold increase in vacuolar proton concentration resulted in a 70-fold increase of the affinity for ATP binding on the cytosolic side. Changes in pH on either side of the membrane seem to be transferred by the V-ATPase to the other side. A mathematical model was developed that indicates a feedback of proton concentration on peak H(+) current amplitude (v(max)) and ATP consumption (K(m)) of the V-ATPase. It proposes that for efficient V-ATPase function dissociation of transported protons from the pump protein might become higher with increasing pH. This feature results in an optimization of H(+) pumping by the V-ATPase according to existing H(+) concentrations.
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