ArticleMolecular & cellular proteomics : MCP2012
Determining degradation and synthesis rates of arabidopsis proteins using the kinetics of progressive 15N labeling of two-dimensional gel-separated protein spots.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Elevated Temperature Effects on Protein Turnover Dynamics inInternational journal of molecular sciences · 2024Article
- Enzymes degraded under high light maintain proteostasis by transcriptional regulation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Subcellular Proteomics as a Unified Approach of Experimental Localizations and Computed Prediction Data for Arabidopsis and Crop Plants.Advances in experimental medicine and biology · 2021Article
- Roles of Reactive Oxygen Species and Mitochondria in Seed Germination.Frontiers in plant science · 2021Review
- Slow Protein Turnover Explains Limited Protein-Level Response to Diurnal Transcriptional Oscillations in Cyanobacteria.Frontiers in microbiology · 2021Article
- Translational gene regulation in plants: A green new deal.Wiley interdisciplinary reviews. RNA · 2020Review
- The composition and turnover of the Arabidopsis thaliana 80S cytosolic ribosome.The Biochemical journal · 2020Article
- Accumulation of "Old Proteins" and the Critical Need for MS-based Protein Turnover Measurements in Aging and Longevity.Proteomics · 2020Review
- A reference-based protein degradation assay without global translation inhibitors.The Journal of biological chemistry · 2017Article
- A Simple Light Isotope Metabolic Labeling (SLIM-labeling) Strategy: A Powerful Tool to Address the Dynamics of Proteome VariationsMolecular & cellular proteomics : MCP · 2017Article
- Spatially resolved metabolic analysis reveals a central role for transcriptional control in carbon allocation to wood.Journal of experimental botany · 2017Article
- Coordinated protein co-expression in plants by harnessing the synergy between an intein and a viral 2A peptide.Plant biotechnology journal · 2017Article
- Protein Degradation Rate inThe Plant cell · 2017Article
- Production of highly bioactive resveratrol analogues pterostilbene and piceatannol in metabolically engineered grapevine cell cultures.Plant biotechnology journal · 2016Article
- Drought and Recovery: Independently Regulated Processes Highlighting the Importance of Protein Turnover Dynamics and Translational Regulation in Medicago truncatula.Molecular & cellular proteomics : MCP · 2016Article
- Proteome Scale-Protein Turnover Analysis Using High Resolution Mass Spectrometric Data from Stable-Isotope Labeled Plants.Journal of proteome research · 2016Article
- A Method of Accounting for Enzyme Costs in Flux Balance Analysis Reveals Alternative Pathways and Metabolite Stores in an Illuminated Arabidopsis Leaf.Plant physiology · 2015Article
- INTERMEDIATE CLEAVAGE PEPTIDASE55 Modifies Enzyme Amino Termini and Alters Protein Stability in Arabidopsis Mitochondria.Plant physiology · 2015Article
- Quantifying protein synthesis and degradation in Arabidopsis by dynamic 13CO2 labeling and analysis of enrichment in individual amino acids in their free pools and in protein.Plant physiology · 2015Article
- Proteins with high turnover rate in barley leaves estimated by proteome analysis combined with in planta isotope labeling.Plant physiology · 2014Article
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5 authors.
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Abstract
The growth and development of plant tissues is associated with an ordered succession of cellular processes that are reflected in the appearance and disappearance of proteins. The control of the kinetics of protein turnover is central to how plants can rapidly and specifically alter protein abundance and thus molecular function in response to environmental or developmental cues. However, the processes of turnover are largely hidden during periods of apparent steady-state protein abundance, and even when proteins accumulate it is unclear whether enhanced synthesis or decreased degradation is responsible. We have used a (15)N labeling strategy with inorganic nitrogen sources coupled to a two-dimensional fluorescence difference gel electrophoresis and mass spectrometry analysis of two-dimensional IEF/SDS-PAGE gel spots to define the rate of protein synthesis (K(S)) and degradation (K(D)) of Arabidopsis cell culture proteins. Through analysis of MALDI-TOF/TOF mass spectra from 120 protein spots, we were able to quantify K(S) and K(D) for 84 proteins across six functional groups and observe over 65-fold variation in protein degradation rates. K(S) and K(D) correlate with functional roles of the proteins in the cell and the time in the cell culture cycle. This approach is based on progressive (15)N labeling that is innocuous for the plant cells and, because it can be used to target analysis of proteins through the use of specific gel spots, it has broad applicability.
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