ArticleParasitology research2012
Cloning and characterization of a novel cathepsin B-like cysteine proteinase from Angiostrongylus cantonensis.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2023Article
- Activity profiling of peptidases in Angiostrongylus costaricensis first-stage larvae and adult worms.PLoS neglected tropical diseases · 2018Article
- Cysteine proteases as digestive enzymes in parasitic helminths.PLoS neglected tropical diseases · 2018Review
- Cloning and expression of a 16-kDa recombinant protein from Angiostrongylus cantonensis for use in immunoblot diagnosis of human angiostrongyliasis.Parasitology research · 2016Article
- Pepsin is a positive regulator of Ac-cathB-2 involved in the rat gut penetration of Angiostrongylus cantonensis.Parasites & vectors · 2016Article
- The cathepsin S cysteine proteinase of the burrowing nematode Radopholus similis is essential for the reproduction and invasion.Cell & bioscience · 2016Article
- Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis.International journal of biological sciences · 2015Article
- Angiostrongylus cantonensis cathepsin B-like protease (Ac-cathB-1) is involved in host gut penetration.Parasite (Paris, France) · 2015Article
- Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis.PLoS neglected tropical diseases · 2015Article
- Analysis of a novel cathepsin B circulating antigen and its response to drug treatment in Trichinella-infected mice.Parasitology research · 2013Article
- Identification and characterisation of microRNAs in young adults of Angiostrongylus cantonensis via a deep-sequencing approach.Memorias do Instituto Oswaldo Cruz · 2013Article
- The mRNA level of the galectin-10 of Angiostrongylus cantonensis induced by reactive oxygen stress.Parasitology research · 2013Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Cysteine protease plays a key role in host-parasite interactions. In this study, we identified a novel gene encoding a cathepsin B-like cysteine protease (AcCBL1) from the cDNA library of Angiostrongysus cantonensis fourth-stage larvae (L4) and characterized its biological role in the parasite. Sequence and phylogeny analysis showed that AcCBL1 is related to other cathepsin B family members with the conserved catalytic triad (Cys, His, Asn) and diagnostic occluding loop. In addition, the sequence contains a specific "hemoglobinase motif" and might have a hemoglobinase (Hb)-degrading function. The recombinant AcCBL1 (rAcCBL1) exhibited the protease activity by gelation SDS/PAGE assay; rAcCBL1 can cleave the fluorogenic substrate Z-Arg-Arg-AMC, and the optimum pH was 5.5. The enzyme can hydrolyse several host proteins including Hb and human IgG in acidic pH, but low levels of hydrolysis were observed in neutral pH. Reverse transcription polymerase chain reaction revealed that AcCBL1 expression was detected throughout various developmental stages, L3, L4, adult male and female worms. Western blotting analysis indicated that AcCBL1 was an excretory/secretory product of L4 in mature form of protease. Immunolocalization demonstrated that AcCBL1 was mainly localized in the intestine of L4. These results suggest that rAcCBL1 may play an important role in the parasite nutrition uptake.
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