ArticlePlant physiology2012
The effect of a genetically reduced plasma membrane protonmotive force on vegetative growth of Arabidopsis.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed, 150 citations in OpenAlex.
- Cellular pH homeostasis shapes root system architecture by modulating auxin-mediated developmental responses.Plant physiology · 2025Article
- Identification and evaluation of low-pH-tolerant Cheongcheong/Nagdong-double haploid rice lines via QTL analysis.BMC plant biology · 2025Article
- Understanding the mechanobiology of phytoacoustics through molecular Lens: Mechanisms and future perspectives.Journal of advanced research · 2024Review
- The brassinosteroid receptor StBRI1 promotes tuber development by enhancing plasma membrane H+-ATPase activity in potato.The Plant cell · 2024Article
- Phototropin2 3'UTR overlaps with the AT5G58150 gene encoding an inactive RLK kinase.BMC plant biology · 2024Article
- Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.PLoS genetics · 2024Article
- Assaying the Effect of Peptide Treatment on HMethods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Article
- PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice.Plant physiology · 2023Article
- Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals.Nature communications · 2023Article
- New Genes Identified as Modulating Salt Tolerance in Maize Seedlings Using the Combination of Transcriptome Analysis and BSA.Plants (Basel, Switzerland) · 2023Article
- The KFrontiers in plant science · 2023Article
- New Insight into Plant Saline-Alkali Tolerance Mechanisms and Application to Breeding.International journal of molecular sciences · 2022Review
- Cell-Type-Specific Length and Cytosolic pH Response of Superficial Cells ofPlants (Basel, Switzerland) · 2022Article
- Review
- The evolution of plant proton pump regulation via the R domain may have facilitated plant terrestrialization.Communications biology · 2022Article
- SAUR63 stimulates cell growth at the plasma membrane.PLoS genetics · 2022Article
- Comparing Essentiality ofInternational journal of molecular sciences · 2022Article
- Receptor-like protein kinase BAK1 promotes K+ uptake by regulating H+-ATPase AHA2 under low potassium stress.Plant physiology · 2022Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plasma membrane proton gradient is an essential feature of plant cells. In Arabidopsis (Arabidopsis thaliana), this gradient is generated by the plasma membrane proton pump encoded by a family of 11 genes (abbreviated as AHA, for Arabidopsis H(+)-ATPase), of which AHA1 and AHA2 are the two most predominantly expressed in seedlings and adult plants. Although double knockdown mutant plants containing T-DNA insertions in both genes are embryonic lethal, under ideal laboratory growth conditions, single knockdown mutant plants with a 50% reduction in proton pump concentration complete their life cycle without any observable growth alteration. However, when grown under conditions that induce stress on the plasma membrane protonmotive force (PMF), such as high external potassium to reduce the electrical gradient or high external pH to reduce the proton chemical gradient, aha2 mutant plants show a growth retardation compared with wild-type plants. In this report, we describe the results of studies that examine in greater detail AHA2's specific role in maintaining the PMF during seedling growth. By comparing the wild type and aha2 mutants, we have measured the effects of a reduced PMF on root and hypocotyl growth, ATP-induced skewed root growth, and rapid cytoplasmic calcium spiking. In addition, genome-wide gene expression profiling revealed the up-regulation of potassium transporters in aha2 mutants, indicating, as predicted, a close link between the PMF and potassium uptake at the plasma membrane. Overall, this characterization of aha2 mutants provides an experimental and theoretical framework for investigating growth and signaling processes that are mediated by PMF-coupled energetics at the cell membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.