ArticleAntimicrobial agents and chemotherapy2012
Lactoferrin inhibits Porphyromonas gingivalis proteinases and has sustained biofilm inhibitory activity.
Article in Antimicrobial agents and chemotherapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Mass Spectrometry-Based Proteomics for Discovering Salivary Biomarkers in Periodontitis: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Review
- Crosstalk between iron metabolism dysregulation and the oral microbiome in periodontitis.Journal of oral microbiology · 2026Review
- Review
- A Comparative Study of the Impact of Chemical Debridement Products on the Proteomic Profile of the Salivary Pellicle and Cell Adhesion on OsseoSpeed Titanium Dental Implant Surfaces.ACS applied materials & interfaces · 2025Article
- Salivary lactoferrin levels in Down Syndrome: a case-control study.Brain, behavior, & immunity - health · 2025Article
- Association of salivary lactoferrin,Frontiers in immunology · 2025Article
- Salivary proteins modulate Candida albicans virulence and may prevent oropharingeal candidiasis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Lactoferrin Affects the Viability of Bacteria in a Biofilm and the Formation of a New Biofilm Cycle ofInternational journal of molecular sciences · 2024Article
- Lactoferrin impairs pathogen virulence through its proteolytic activity.Frontiers in veterinary science · 2024Review
- MAD-microbial (origin of) Alzheimer's disease hypothesis: from infection and the antimicrobial response to disruption of key copper-based systems.Frontiers in neuroscience · 2024Review
- Implication of salivary lactoferrin and periodontal-mediated infections in Alzheimer's disease.Neural regeneration research · 2023Article
- Therapeutic potential of lactoferrin-coated iron oxide nanospheres for targeted hyperthermia in gastric cancer.Scientific reports · 2023Article
- Synergistic antibacterial effects of 5-fluorouracil or thioridazine in combination with phytopolyphenols on culturedJournal of dental sciences · 2023Article
- Time to Kill and Time to Heal: The Multifaceted Role of Lactoferrin and Lactoferricin in Host Defense.Pharmaceutics · 2023Review
- Bovine Milk and Yogurt Affect Oral Microorganisms and Biofilms In-Vitro.Oral health & preventive dentistry · 2023Article
- The role of microbiome-host interactions in the development of Alzheimer´s disease.Frontiers in cellular and infection microbiology · 2023Review
- Novel regimens of phytopolyphenols with cisplatin or memantine and ZnSOJournal of dental sciences · 2022Article
- Regulation of zinc-dependent enzymes by metal carrier proteins.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2022Review
- Characterization of proteolytic degradation products of vaginally administered bovine lactoferrin.PloS one · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porphyromonas gingivalis is a bacterial pathogen associated with chronic periodontitis that results in destruction of the tooth's supporting tissues. The major virulence determinants of P. gingivalis are its cell surface Arg- and Lys-specific cysteine proteinases, RgpA/B and Kgp. Lactoferrin (LF), an 80-kDa iron-binding glycoprotein found in saliva and gingival crevicular fluid, is believed to play an important role in innate immunity. In this study, bovine milk LF displayed proteinase inhibitory activity against P. gingivalis whole cells, significantly inhibiting both Arg- and Lys-specific proteolytic activities. LF inhibited the Arg-specific activity of purified RgpB, which lacks adhesin domains, and also inhibited the same activity of the RgpA/Kgp proteinase-adhesin complexes in a time-dependent manner, with a first-order inactivation rate constant (k(inact)) of 0.023 min(-1) and an inhibitor affinity constant (K(I)) of 5.02 μM. LF inhibited P. gingivalis biofilm formation by >80% at concentrations above 0.625 μM. LF was relatively resistant to hydrolysis by P. gingivalis cells but was cleaved into two major polypeptides (53 and 33 kDa) at R(284) to S(285), as determined by in-source decay mass spectrometry; however, these polypeptides remained associated with each other and retained inhibitory activity. The biofilm inhibitory activity of LF against P. gingivalis was not attributed to direct antibacterial activity, as LF displayed little growth inhibitory activity against planktonic cells. As the known RgpA/B and Kgp inhibitor N-α-p-tosyl-l-lysine chloromethylketone also inhibited P. gingivalis biofilm formation, the antibiofilm effect of LF may at least in part be attributable to its antiproteinase activity.
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Registered trials
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