ArticleParasites & vectors2012
Quantitative PCR-based genome size estimation of the astigmatid mites Sarcoptes scabiei, Psoroptes ovis and Dermatophagoides pteronyssinus.
Article in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Scabies.Nature reviews. Disease primers · 2024Review
- Diseases of Iberian ibex (European journal of wildlife research · 2023Review
- Proteomic analysis of Sarcoptes scabiei reveals that proteins differentially expressed between eggs and female adult stages are involved predominantly in genetic information processing, metabolism and/or host-parasite interactions.PLoS neglected tropical diseases · 2022Article
- Evidence that Transcriptional Alterations inInternational journal of molecular sciences · 2022Article
- An RNA Interference Tool to Silence Genes inInternational journal of molecular sciences · 2022Article
- Dermoscopy, light microscopy, and real-time polymerase chain reaction for the diagnosis of scabies. Preliminary results.Postepy dermatologii i alergologii · 2021Article
- High-quality nuclear genome for Sarcoptes scabiei-A critical resource for a neglected parasite.PLoS neglected tropical diseases · 2020Article
- The genome, transcriptome, and proteome of the fish parasite Pomphorhynchus laevis (Acanthocephala).PloS one · 2020Article
- Gene silencing by RNA interference in Sarcoptes scabiei: a molecular tool to identify novel therapeutic targets.Parasites & vectors · 2017Article
- Draft genome of the honey bee ectoparasitic mite, Tropilaelaps mercedesae, is shaped by the parasitic life history.GigaScience · 2017Article
- Draft genome of the scabies mite.Parasites & vectors · 2015Article
- Evolution of parasitism along convergent lines: from ecology to genomics.Parasitology · 2015Review
- Estimation of the genome sizes of the chigger mites Leptotrombidium pallidum and Leptotrombidium scutellare based on quantitative PCR and k-mer analysis.Parasites & vectors · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lack of genomic data available for mites limits our understanding of their biology. Evolving high-throughput sequencing technologies promise to deliver rapid advances in this area, however, estimates of genome size are initially required to ensure sufficient coverage.
methodsQuantitative real-time PCR was used to estimate the genome sizes of the burrowing ectoparasitic mite Sarcoptes scabiei, the non-burrowing ectoparasitic mite Psoroptes ovis, and the free-living house dust mite Dermatophagoides pteronyssinus. Additionally, the chromosome number of S. scabiei was determined by chromosomal spreads of embryonic cells derived from single eggs.
resultsS. scabiei cells were shown to contain 17 or 18 small (< 2 μM) chromosomes, suggesting an XO sex-determination mechanism. The average estimated genome sizes of S. scabiei and P. ovis were 96 (± 7) Mb and 86 (± 2) Mb respectively, among the smallest arthropod genomes reported to date. The D. pteronyssinus genome was estimated to be larger than its parasitic counterparts, at 151 Mb in female mites and 218 Mb in male mites.
conclusionsThis data provides a starting point for understanding the genetic organisation and evolution of these astigmatid mites, informing future sequencing projects. A comparitive genomic approach including these three closely related mites is likely to reveal key insights on mite biology, parasitic adaptations and immune evasion.
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