ArticleParasites & vectors2012
Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice.
Article in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 103 citations in OpenAlex.
- Loop-Mediated Isothermal Amplification (LAMP) as a Rapid Molecular Tool for Salmonella Detection in Food.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Evolution ofJournal of parasitology research · 2026Review
- Recent advances in isothermal amplification techniques for detection of animal diseases.Frontiers in veterinary science · 2026Review
- Review of Toxoplasmosis: What We Still Need to Do.Veterinary sciences · 2025Review
- Development of a LAMP method with lateral flow DNA chromatography to diagnose toxoplasmosis in immunocompromised patients.Tropical medicine and health · 2024Article
- Seromolecular study on the prevalence and risk factors of Toxoplasma gondii infection in pregnant women referred to a gynecology hospital in Urmia, northwest part of Iran in 2022.BMC infectious diseases · 2024Article
- Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus.Microbiology spectrum · 2022Article
- Utility of blood as the clinical specimen for the diagnosis of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification and real-time polymerase chain reaction assays based on REP-529 sequence and B1 gene.BMC infectious diseases · 2022Article
- A novel rapid visual detection assay for Toxoplasma gondii combining recombinase-aided amplification and lateral flow dipstick coupled with CRISPR-Cas13a fluorescence (RAA-Cas13a-LFD).Parasite (Paris, France) · 2022Article
- Detection and genetic characterisation of Toxoplasma gondii circulating in free-range chickens, pigs and seropositive pregnant women in Benue state, Nigeria.PLoS neglected tropical diseases · 2021Article
- Toxoplasma gondii in Chickens (Gallus domesticus) from North India.Acta parasitologica · 2021Article
- Design of Rapid Detection System for Five Major Carbapenemase Families (Infection and drug resistance · 2021Article
- A novel loop-mediated isothermal amplification-lateral-flow-dipstick (LAMP-LFD) device for rapid detection of Toxoplasma gondii in the blood of stray cats and dogs.Parasite (Paris, France) · 2021Article
- Validation of a Commercial Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection ofFoods (Basel, Switzerland) · 2020Article
- A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV.Biochip journal · 2019Article
- Validation of SYBR green I based closed tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL).PLoS neglected tropical diseases · 2018Article
- Advances in serological, imaging techniques and molecular diagnosis of Toxoplasma gondii infection.Infection · 2018Review
- Development of Visually Improved Loop Mediated Isothermal Amplification for the Diagnosis ofThe American journal of tropical medicine and hygiene · 2018Article
- Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.Sensors and actuators. B, Chemical · 2018Review
- APeerJ · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundToxoplasmosis is a widespread zoonotic parasitic disease that occurs in both animals and humans. Traditional molecular assays are often difficult to perform, especially for the early diagnosis of Toxoplasma gondii infections. Here, we established a novel loop-mediated isothermal amplification targeting the 529 bp repeat element (529 bp-LAMP) to detect T. gondii DNA in blood samples of experimental mice infected with tachyzoites of the RH strain.
findingsThe assay was performed with Bst DNA polymerase at 65°C for 1 h. The detection limit of the 529 bp-LAMP assay was as low as 0.6 fg of T. gondii DNA. The sensitivity of this assay was 100 and 1000 fold higher than that of the LAMP targeting B1 gene (B1-LAMP) and nested PCR targeting 529 bp repeat element (529 bp-nested PCR), respectively. The specificity of the 529 bp-LAMP assay was determined using the DNA samples of Trypanosoma evansi, Plasmodium falciparum, Paragonimus westermani, Schistosoma japonicum, Fasciola hepatica and Angiostrongylus cantonensis. No cross-reactivity with the DNA of any parasites was found. The assay was able to detect T. gondii DNA in all mouse blood samples at one day post infection (dpi).
conclusionsWe report the following findings: (i) The detection limit of the 529 bp-LAMP assay is 0.6 fg of T. gondii DNA; (ii) The assay does not involve any cross-reactivity with the DNA of other parasites; (iii) This is the first report on the application of the LAMP assay for early diagnosis of toxoplasmosis in blood samples from experimentally infected mice. Due to its simplicity, sensitivity and cost-effectiveness for common use, we suggest that this assay should be used as an early diagnostic tool for health control of toxoplasmosis.
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