ArticleMicrobial cell factories2012
Laboratory evolution of copper tolerant yeast strains.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- Enabling malic acid production from corn-stover hydrolysate in Lipomyces starkeyi via metabolic engineering and bioprocess optimization.Microbial cell factories · 2025Article
- A Droplet-Based Microfluidic Platform for High-Throughput Culturing of Yeast Cells in Various Conditions.Micromachines · 2024Article
- Simultaneous bioreduction of tellurite and selenite by Yarrowia lipolytica, Trichosporon cutaneum, and their co-culture along with characterization of biosynthesized Te-Se nanoparticles.Microbial cell factories · 2023Article
- Epigenetic architecture ofEcology and evolution · 2023Article
- Axenic Long-Term Cultivation ofJournal of fungi (Basel, Switzerland) · 2023Article
- Rhodotorula mucilaginosa YR29 is able to accumulate PbWorld journal of microbiology & biotechnology · 2023Article
- Copper Accumulation Efficiency in Different Recombinant Microorganism Strains Available for Bioremediation of Heavy Metal-Polluted Waters.International journal of molecular sciences · 2023Article
- A Comparative Study on Heavy Metal Removal from CCA-Treated Wood Waste byJournal of fungi (Basel, Switzerland) · 2023Article
- Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach.Journal of fungi (Basel, Switzerland) · 2023Article
- Genomic Adaptations ofMicroorganisms · 2022Review
- Enhancement of Copper Uptake of Yeast Through Systematic Optimization of Medium and the Cultivation Process of Saccharomyces cerevisiae.Applied biochemistry and biotechnology · 2022Article
- Interspecific hybrids show a reduced adaptive potential under DNA damaging conditions.Evolutionary applications · 2021Article
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- Heavy Metal Tolerance of NovelMycobiology · 2020Article
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- Toxicity, Physiological, and Ultrastructural Effects of Arsenic and Cadmium on the Extremophilic MicroalgaInternational journal of environmental research and public health · 2020Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Heavy metal tolerance in marine strains of Yarrowia lipolytica.Extremophiles : life under extreme conditions · 2018Article
- Genome plasticity in response to stress in Tetrahymena thermophila: selective and reversible chromosome amplification and paralogous expansion of metallothionein genes.Environmental microbiology · 2018Article
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5 authors.
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Abstract
backgroundYeast strains endowed with robustness towards copper and/or enriched in intracellular Cu might find application in biotechnology processes, among others in the production of functional foods. Moreover, they can contribute to the study of human diseases related to impairments of copper metabolism. In this study, we investigated the molecular and physiological factors that confer copper tolerance to strains of baker's yeasts.
resultsWe characterized the effects elicited in natural strains of Candida humilis and Saccharomyces cerevisiae by the exposure to copper in the culture broth. We observed that, whereas the growth of Saccharomyces cells was inhibited already at low Cu concentration, C. humilis was naturally robust and tolerated up to 1 g · L-1 CuSO4 in the medium. This resistant strain accumulated over 7 mg of Cu per gram of biomass and escaped severe oxidative stress thanks to high constitutive levels of superoxide dismutase and catalase. Both yeasts were then "evolved" to obtain hyper-resistant cells able to proliferate in high copper medium. While in S. cerevisiae the evolution of robustness towards Cu was paralleled by the increase of antioxidative enzymes, these same activities decreased in evolved hyper-resistant Candida cells. We also characterized in some detail changes in the profile of copper binding proteins, that appeared to be modified by evolution but, again, in a different way in the two yeasts.
conclusionsFollowing evolution, both Candida and Saccharomyces cells were able to proliferate up to 2.5 g · L-1 CuSO4 and to accumulate high amounts of intracellular copper. The comparison of yeasts differing in their robustness, allowed highlighting physiological and molecular determinants of natural and acquired copper tolerance. We observed that different mechanisms contribute to confer metal tolerance: the control of copper uptake, changes in the levels of enzymes involved in oxidative stress response and changes in the copper-binding proteome. However, copper elicits different physiological and molecular reactions in yeasts with different backgrounds.
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