ArticleJournal of experimental botany2012
A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana.
Article in Journal of experimental botany, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 78 citations in OpenAlex.
- Perturbation of mitochondrial CaStress biology · 2026Article
- The VAMP-associated protein VAP27-1 plays a crucial role in plant resistance to ER stress by modulating ER-PM contact architecture in Arabidopsis.Plant communications · 2024Article
- A developmentally controlled cellular decompartmentalization process executes programmed cell death in the Arabidopsis root cap.The Plant cell · 2024Article
- SCAB1 coordinates sequential CaScience China. Life sciences · 2024Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Quantitative Analysis of Plant Cytosolic Calcium Signals in Response to Water Activated by Low-Power Non-Thermal Plasma.International journal of molecular sciences · 2022Article
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments.Plant physiology · 2022Article
- Monitoring calcium handling by the plant endoplasmic reticulum with a low-CaThe Plant journal : for cell and molecular biology · 2022Article
- Sensing Mechanisms: Calcium Signaling Mediated Abiotic Stress in Plants.Frontiers in plant science · 2022Review
- A Plastid-Bound Ankyrin Repeat Protein Controls Gametophyte and Early Embryo Development inFrontiers in plant science · 2022Article
- Two plastid POLLUX ion channel-like proteins are required for stress-triggered stromal Ca2+release.Plant physiology · 2021Article
- Plasma-Activated Water Triggers Rapid and Sustained Cytosolic CaPlants (Basel, Switzerland) · 2021Article
- Illuminating the hidden world of calcium ions in plants with a universe of indicators.Plant physiology · 2021Review
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- Stromal NADH supplied by PHOSPHOGLYCERATE DEHYDROGENASE3 is crucial for photosynthetic performance.Plant physiology · 2021Article
- Chloroplast Calcium Signaling in the Spotlight.Frontiers in plant science · 2020Review
- Identification of Novel Inhibitors of Auxin-Induced CaPlant physiology · 2019Article
- The High Light Response in Arabidopsis Requires the Calcium Sensor Protein CAS, a Target of STN7- and STN8-Mediated Phosphorylation.Frontiers in plant science · 2019Article
- A Tonoplast-Associated Calcium-Signaling Module Dampens ABA Signaling during Stomatal Movement.Plant physiology · 2018Article
- Chloroplast CaPlant physiology · 2018Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Calcium has long been acknowledged as one of the most important signalling components in plants. Many abiotic and biotic stimuli are transduced into a cellular response by temporal and spatial changes in cellular calcium concentration and the calcium-sensitive protein aequorin has been exploited as a genetically encoded calcium indicator for the measurement of calcium in planta. The objective of this work was to generate a compatible set of aequorin expression plasmids for the generation of transgenic plant lines to measure changes in calcium levels in different cellular subcompartments. Aequorin was fused to different targeting peptides or organellar proteins as a means to localize it to the cytosol, the nucleus, the plasma membrane, and the mitochondria. Furthermore, constructs were designed to localize aequorin in the stroma as well as the inner and outer surface of the chloroplast envelope membranes. The modular set-up of the plasmids also allows the easy replacement of targeting sequences to include other compartments. An additional YFP-fusion was included to verify the correct subcellular localization of all constructs by laser scanning confocal microscopy. For each construct, pBin19-based binary expression vectors driven by the 35S or UBI10 promoter were made for Agrobacterium-mediated transformation. Stable Arabidopsis lines were generated and initial tests of several lines confirmed their feasibility to measure calcium signals in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.