ArticleChemphyschem : a European journal of chemical physics and physical chemistry2012
Multicolor super-resolution fluorescence imaging via multi-parameter fluorophore detection.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Herpes simplex virus type 1 reshapes host chromatin architecture via transcription machinery hijacking.Nature communications · 2025Article
- Blinking characteristics of organic fluorophores for blink-based multiplexing.Communications chemistry · 2024Article
- RegiSTORM: channel registration for multi-color stochastic optical reconstruction microscopy.BMC bioinformatics · 2023Article
- Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.Scientific reports · 2023Article
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Review
- Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.International journal of molecular sciences · 2022Article
- Imaging the endocannabinoid signaling system.Journal of neuroscience methods · 2022Review
- Multiplexed structured illumination super-resolution imaging with lifetime-engineered upconversion nanoparticles.Nanoscale advances · 2021Article
- Toward photoswitchable electronic pre-resonance stimulated Raman probes.The Journal of chemical physics · 2021Article
- Serial imaging of micro-agents and cancer cell spheroids in a microfluidic channel using multicolor fluorescence microscopy.PloS one · 2021Article
- Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.Journal of the American Chemical Society · 2020Review
- Spectral cross-cumulants for multicolor super-resolved SOFI imaging.Nature communications · 2020Article
- Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells.Science (New York, N.Y.) · 2020Article
- Innovative particle standards and long-lived imaging for 2D and 3D dSTORM.Scientific reports · 2019Article
- Article
- Multicolor re-scan super-resolution imaging of live cells.Quantitative imaging in medicine and surgery · 2019Article
- Article
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4 authors.
Funding
Abstract
Understanding the complexity of the cellular environment will benefit from the ability to unambiguously resolve multiple cellular components, simultaneously and with nanometer-scale spatial resolution. Multicolor super-resolution fluorescence microscopy techniques have been developed to achieve this goal, yet challenges remain in terms of the number of targets that can be simultaneously imaged and the crosstalk between color channels. Herein, we demonstrate multicolor stochastic optical reconstruction microscopy (STORM) based on a multi-parameter detection strategy, which uses both the fluorescence activation wavelength and the emission color to discriminate between photo-activatable fluorescent probes. First, we obtained two-color super-resolution images using the near-infrared cyanine dye Alexa 750 in conjunction with a red cyanine dye Alexa 647, and quantified color crosstalk levels and image registration accuracy. Combinatorial pairing of these two switchable dyes with fluorophores which enhance photo-activation enabled multi-parameter detection of six different probes. Using this approach, we obtained six-color super-resolution fluorescence images of a model sample. The combination of multiple fluorescence detection parameters for improved fluorophore discrimination promises to substantially enhance our ability to visualize multiple cellular targets with sub-diffraction-limit resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.