ArticleBiotechnology for biofuels2012
A versatile toolkit for high throughput functional genomics with Trichoderma reesei.
Article in Biotechnology for biofuels, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 841 citations in OpenAlex.
- Heterologous Expression of aJournal of fungi (Basel, Switzerland) · 2026Article
- Plant recognition by Trichoderma Harzianum elicits upregulation of a novel secondary metabolite cluster required for colonization.Scientific reports · 2026Article
- The Biocontrol and Growth-Promoting Potential ofPlants (Basel, Switzerland) · 2025Article
- N-acetylglucosamine sensing in the filamentous soil fungus Trichoderma reesei.The FEBS journal · 2025Article
- Enhanced heterologous gene expression in Trichoderma reesei by promoting multicopy integration.Applied microbiology and biotechnology · 2024Article
- The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei.Scientific reports · 2024Article
- Engineering of glycoside hydrolase family 7 cellobiohydrolases directed by natural diversity screening.The Journal of biological chemistry · 2024Article
- RGS4 impacts carbohydrate and siderophore metabolism in Trichoderma reesei.BMC genomics · 2023Article
- Current genetic strategies to investigate gene functions in Trichoderma reesei.Microbial cell factories · 2023Review
- High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer brewing.Microbial cell factories · 2023Article
- MAPkinases regulate secondary metabolism, sexual development and light dependent cellulase regulation in Trichoderma reesei.Scientific reports · 2023Article
- Characterization of Recombinant Antimicrobial Peptide BMGlv2 Heterologously Expressed inInternational journal of molecular sciences · 2022Article
- Review
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Molecular methods unravel the biosynthetic potential ofRSC advances · 2021Review
- Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei.Microbial cell factories · 2021Article
- Genetic Transformation of Trichoderma spp.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- An efficient transformation system for Trichoderma atroviride using the pyr4 gene as a selectable marker.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Chitin and chitosan remodeling defines vegetative development and Trichoderma biocontrol.PLoS pathogens · 2020Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina), represents a biotechnological workhorse and is currently one of the most proficient cellulase producers. While strain improvement was traditionally accomplished by random mutagenesis, a detailed understanding of cellulase regulation can only be gained using recombinant technologies.
resultsAiming at high efficiency and high throughput methods, we present here a construction kit for gene knock out in T. reesei. We provide a primer database for gene deletion using the pyr4, amdS and hph selection markers. For high throughput generation of gene knock outs, we constructed vectors using yeast mediated recombination and then transformed a T. reesei strain deficient in non-homologous end joining (NHEJ) by spore electroporation. This NHEJ-defect was subsequently removed by crossing of mutants with a sexually competent strain derived from the parental strain, QM9414.
conclusionsUsing this strategy and the materials provided, high throughput gene deletion in T. reesei becomes feasible. Moreover, with the application of sexual development, the NHEJ-defect can be removed efficiently and without the need for additional selection markers. The same advantages apply for the construction of multiple mutants by crossing of strains with different gene deletions, which is now possible with considerably less hands-on time and minimal screening effort compared to a transformation approach. Consequently this toolkit can considerably boost research towards efficient exploitation of the resources of T. reesei for cellulase expression and hence second generation biofuel production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.