ArticleNucleic acids research2012
A non-canonical E-box within the MyoD core enhancer is necessary for circadian expression in skeletal muscle.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 62 citations in OpenAlex.
- Circadian biology and exercise: the time to move.Life metabolism · 2026Review
- Co-expression network analysis reveals repression ofbioRxiv : the preprint server for biology · 2026Article
- New insights into the skeletal muscle circadian clock in ruminants.Journal of animal science and biotechnology · 2026Review
- Resveratrol and dexamethasone have cell-specific effects on the circadian clock but not on the rhythm of mitochondrial function in the fetal heart.Journal of physiology and biochemistry · 2026Article
- Immediate nuclear accumulation of BMAL1 to regulate cellular circadian clock synchronization.Communications biology · 2025Article
- Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves.Animal bioscience · 2025Article
- Ablation of satellite cell-specific clock gene, Bmal1, alters force production, muscle damage, and repair following contractile-induced injury.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Circadian Rhythm and Muscle Function.Advances in experimental medicine and biology · 2025Review
- Circadian regulation of endoplasmic reticulum calcium response in cultured mouse astrocytes.eLife · 2024Article
- Causal Associations Between Sleep Traits and Low Grip Strength: A Bidirectional Mendelian Randomization Study.Nature and science of sleep · 2024Article
- Potential role of Akt in the regulation of fibroblast growth factor 21 by berberine.Journal of natural medicines · 2024Article
- Article
- Molecular clocks, satellite cells, and skeletal muscle regeneration.American journal of physiology. Cell physiology · 2023Review
- The circadian E3 ligase FBXL21 regulates myoblast differentiation and sarcomere architecture via MYOZ1 ubiquitination and NFAT signaling.PLoS genetics · 2022Article
- BMAL1 drives muscle repair through control of hypoxic NADGenes & development · 2022Article
- Circadian clocks regulate cardiac arrhythmia susceptibility, repolarization, and ion channels.Current opinion in pharmacology · 2021Review
- The core circadian component, Bmal1, is maintained in the pineal gland of old killifish brain.iScience · 2021Article
- The GSK-3β-FBXL21 Axis Contributes to Circadian TCAP Degradation and Skeletal Muscle Function.Cell reports · 2020Article
- Ticking for Metabolic Health: The Skeletal-Muscle Clocks.Obesity (Silver Spring, Md.) · 2020Review
- Master control: transcriptional regulation of mammalian Myod.Journal of muscle research and cell motility · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The myogenic differentiation 1 (MyoD) gene is a master regulator of myogenesis. We previously reported that the expression of MyoD mRNA oscillates over 24 h in skeletal muscle and that the circadian clock transcription factors, BMAL1 (brain and muscle ARNT-like 1) and CLOCK (circadian locomotor output cycles kaput), were bound to the core enhancer (CE) of the MyoD gene in vivo. In this study, we provide in vivo and in vitro evidence that the CE is necessary for circadian expression of MyoD in adult muscle. Gel shift assays identified a conserved non-canonical E-box within the CE that is bound by CLOCK and BMAL1. Functional analysis revealed that this E-box was required for full activation by BMAL1/CLOCK and for in vitro circadian oscillation. Expression profiling of muscle of CE(loxP/loxP) mice found approximately 1300 genes mis-expressed relative to wild-type. Based on the informatics results, we analyzed the respiratory function of mitochondria isolated from wild-type and CE(loxP/loxP) mice. These assays determined that State 5 respiration was significantly reduced in CE(loxP/loxP) muscle. The results of this work identify a novel element in the MyoD enhancer that confers circadian regulation to MyoD in skeletal muscle and suggest that loss of circadian regulation leads to changes in myogenic expression and downstream mitochondrial function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.