ArticleEukaryotic cell2012
Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Early lipid-mediated responses to hyperosmotic stress at the yeast vacuole.Molecular biology of the cell · 2026Article
- Comparative assessment of food-grade osmolytes for enhancing yeast fermentation performance under salt stress.Microbiology spectrum · 2026Article
- Med3-mediated NADPH generation to helpApplied and environmental microbiology · 2024Article
- Tender love and disassembly: How a TLDc domain protein breaks the V-ATPase.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- Chimeric a-subunit isoforms generate functional yeast V-ATPases with altered regulatory properties in vitro and in vivo.Molecular biology of the cell · 2023Article
- Coordinated conformational changes in the VNature structural & molecular biology · 2022Article
- Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Potassium and Sodium Salt Stress Characterization in the Yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, andApplied and environmental microbiology · 2021Article
- Phosphatidylinositol 3,5-bisphosphate regulates CaTraffic (Copenhagen, Denmark) · 2020Article
- Regulation of V-ATPase Activity and Organelle pH by Phosphatidylinositol Phosphate Lipids.Frontiers in cell and developmental biology · 2020Review
- Interaction of the late endo-lysosomal lipid PI(3,5)P2 with the Vph1 isoform of yeast V-ATPase increases its activity and cellular stress tolerance.The Journal of biological chemistry · 2019Article
- Effects of Disruption of PMC1 in the tfp1∆/∆ Mutant on Calcium Homeostasis, Oxidative and Osmotic Stress Resistance in Candida albicans.Mycopathologia · 2018Article
- Ask yeast how to burn your fats: lessons learned from the metabolic adaptation to salt stress.Current genetics · 2018Review
- Early protection to stress mediated by CDK-dependent PI3,5PThe Journal of cell biology · 2017Article
- Breaking up and making up: The secret life of the vacuolar HProtein science : a publication of the Protein Society · 2017Review
- Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway.The Journal of biological chemistry · 2017Article
- Genetic Causes of Phenotypic Adaptation to the Second Fermentation of Sparkling Wines inG3 (Bethesda, Md.) · 2017Article
- Article
- Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress.Molecular ecology · 2016Article
- Proton Transport and pH Control in Fungi.Advances in experimental medicine and biology · 2016Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Hyperosmotic stress activates an array of cellular detoxification mechanisms, including the high-osmolarity glycerol (HOG) pathway. We report here that vacuolar H(+)-ATPase (V-ATPase) activity helps provide osmotic tolerance in Saccharomyces cerevisiae. V-ATPase subunit genes exhibit complex haploinsufficiency interactions with HOG pathway components. vma mutants lacking V-ATPase function are sensitive to high concentrations of salt and exhibit Hog1p activation even at low salt concentrations, as demonstrated by phosphorylation of Hog1p, a shift in Hog1-green fluorescent protein localization, transcriptional activation of a subset of HOG pathway effectors, and transcriptional inhibition of parallel mitogen-activated protein kinase pathway targets. vma2Δ hog1Δ and vma3Δ pbs2Δ double mutants have a synthetic growth phenotype, poor salt tolerance, and an aberrant, hyper-elongated morphology on solid media, accompanied by activation of a filamentous response element-LacZ construct, indicating cross talk into the filamentous growth pathway. Vacuoles isolated from wild-type cells briefly exposed to salt show higher levels of V-ATPase activity, and Na(+)/H(+) exchange in isolated vacuolar vesicles suggests a biochemical basis for the genetic interactions observed. V-ATPase activity is upregulated during salt stress by increasing assembly of the catalytic V(1) sector with the membrane-bound V(o) sector. Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification.
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