ArticleEukaryotic cell2012
Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Metabolic engineering of Saccharomyces cerevisiae for neoxanthin production.Microbial cell factories · 2025Article
- The galactokinase enzyme of yeast senses metabolic flux to stabilize galactose pathway regulation.Nature metabolism · 2025Article
- A double role of the Gal80 N terminus in activation of transcription by Gal4p.Life science alliance · 2020Article
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- A semi-synthetic regulon enables rapid growth of yeast on xylose.Nature communications · 2018Article
- Genetic and Epigenetic Strategies Potentiate Gal4 Activation to Enhance Fitness in Recently Diverged Yeast Species.Current biology : CB · 2017Article
- A living vector field reveals constraints on galactose network induction in yeast.Molecular systems biology · 2017Article
- Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription.Molecular cell · 2015Article
- Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast.Molecular and cellular biology · 2015Article
- Gene duplication and the evolution of moonlighting proteins.Frontiers in genetics · 2015Review
- DNA Topoisomerases Are Required for Preinitiation Complex Assembly during GAL Gene Activation.PloS one · 2015Article
- The yeast galactose network as a quantitative model for cellular memory.Molecular bioSystems · 2015Review
- Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.FEMS microbiology reviews · 2014Review
- Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch.Molecular and cellular biology · 2013Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Synergistic dual positive feedback loops established by molecular sequestration generate robust bimodal response.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and a ligand sensor (Gal3p)--control the switch between inert and active gene expression. The molecular mechanism by which the recognition of galactose within the cell is converted into a transcriptional response has been the subject of considerable debate. In this study, using a novel and powerful method of localizing active transcription factors within the nuclei of cells, we show that a short-lived complex between Gal4p, Gal80p, and Gal3p occurs soon after the addition of galactose to cells to activate GAL gene expression. Gal3p is subsequently replaced in this complex by Gal1p, and a Gal4p-Gal80p-Gal1p complex is responsible for the continued expression of the GAL genes. The transient role of the ligand sensor indicates that current models for the induction and continued expression of the yeast GAL genes need to be reevaluated.
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