ArticleJournal of bacteriology2012
Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed, 87 citations in OpenAlex.
- Iron and peroxide regulation of the PrrF sRNAs and a conserved ferritin family protein inJournal of bacteriology · 2026Article
- The impact of freezing temperatures on soft tissue and microbial decomposition using human and porcine remains: a pilot study.Forensic science, medicine, and pathology · 2026Article
- Mutations in the mucA gene of Pseudomonas aeruginosa promote disease exacerbation.Archives of microbiology · 2026Review
- Iron and peroxide regulation of the PrrF sRNAs and a conserved Dps-like protein inbioRxiv : the preprint server for biology · 2025Article
- Pseudomonas aeruginosa biofilm: treatment strategies to combat infection.Archives of microbiology · 2025Review
- Fc-modification of anti-PcrV gene-encoded antibodies modulates complement-mediated killing of Pseudomonas aeruginosa.Frontiers in immunology · 2025Article
- Advances in alginate biosynthesis: regulation and production inFrontiers in bioengineering and biotechnology · 2025Review
- Systems serology in cystic fibrosis: Anti-Pseudomonas IgG1 responses and reduced lung function.Cell reports. Medicine · 2023Article
- Transcriptional Regulators Controlling Virulence inInternational journal of molecular sciences · 2023Review
- In Vivo Role of Two-Component Regulatory Systems in Models of Urinary Tract Infections.Pathogens (Basel, Switzerland) · 2023Review
- Mechanisms regulating the CRISPR-Cas systems.Frontiers in microbiology · 2023Review
- Challenges in using transcriptome data to study the c-di-GMP signaling network inFEMS microbes · 2023Article
- Mechanisms of chlorate toxicity and resistance in Pseudomonas aeruginosa.Molecular microbiology · 2022Article
- Article
- RpoN-Based stapled peptides with improved DNA binding suppressRSC medicinal chemistry · 2022Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- Pseudomonas aeruginosa transcriptome adaptations from colonization to biofilm infection of skin wounds.Scientific reports · 2021Article
- Article
- From genotype to phenotype: adaptations ofMicrobial genomics · 2021Review
- Involvement of RpoN in Regulating Motility, Biofilm, Resistance, and Spoilage Potential ofFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Alginate overproduction by Pseudomonas aeruginosa, also known as mucoidy, is associated with chronic endobronchial infections in cystic fibrosis. Alginate biosynthesis is initiated by the extracytoplasmic function sigma factor (σ(22); AlgU/AlgT). In the wild-type (wt) nonmucoid strains, such as PAO1, AlgU is sequestered to the cytoplasmic membrane by the anti-sigma factor MucA that inhibits alginate production. One mechanism underlying the conversion to mucoidy is mutation of mucA. However, the mucoid conversion can occur in wt mucA strains via the degradation of MucA by activated intramembrane proteases AlgW and/or MucP. Previously, we reported that the deletion of the sensor kinase KinB in PAO1 induces an AlgW-dependent proteolysis of MucA, resulting in alginate overproduction. This type of mucoid induction requires the alternate sigma factor RpoN (σ(54)). To determine the RpoN-dependent KinB regulon, microarray and proteomic analyses were performed on a mucoid kinB mutant and an isogenic nonmucoid kinB rpoN double mutant. In the kinB mutant of PAO1, RpoN controlled the expression of approximately 20% of the genome. In addition to alginate biosynthetic and regulatory genes, KinB and RpoN also control a large number of genes including those involved in carbohydrate metabolism, quorum sensing, iron regulation, rhamnolipid production, and motility. In an acute pneumonia murine infection model, BALB/c mice exhibited increased survival when challenged with the kinB mutant relative to survival with PAO1 challenge. Together, these data strongly suggest that KinB regulates virulence factors important for the development of acute pneumonia and conversion to mucoidy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.